8.Working with the Instrument
A few rules:
•Always begin at a low extraction level, then slowly increase it.
•Do not use high extraction levels unless absolutely necessary.
•Different specimen sizes require different extraction levels.
•The faster the trimming or sectioning speed, the lower the extraction
level | should be. |
•The larger and/or thicker the specimen to be trimmed, the lower the extraction value.
•When section specimens with a diameter of 0.5 cm, the
Trimming, 1 x 1cm specimen |
| |
Trimming thickness | Speed | Extract. value |
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10μm | 40% | 1.5 |
10μm | 60% | 1.5 |
10μm | 80% | 1 |
10μm | 100% | 1 |
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20μm | 40% | 2 |
20μm | 60% | 2 |
20μm | 80% | 1.5 |
20μm | 100% | 1 |
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30μm | 40% | 2.5 |
30μm | 60% | 2.5 |
30μm | 80% | 2 |
30μm | 100% | 1.5 |
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40μm | 40% | 3 |
40μm | 60% | 3 |
40μm | 80% | 2.5 |
40μm | 100% | 2 |
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Trimming, 1 x 1cm specimen |
| |
Trimming thickness | Speed | Extract. value |
|
|
|
1μm - 5 μm | 40% | 2.5 |
| 60% | 2 |
| 80% | 1.5 |
| 100% | 1.5 |
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10μm | 40% | 2.5 |
| 60% | 2 |
| 80% | 2 |
| 100% | 1.5 |
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The values listed here are possible settings that depend on the tissue type, specimen size, section thick- ness and section speed, and are in- tended for orientation purposes only.
These settings can also vary when using different
46 | Operating Manual V1.1 – 05/2007 |