Cryofect, VPE Leica Cryofect 4x 350ml | Leica CM1950 guide

11.Optional Accessories

Section waste tray	0477 40062
Brush shelf	0477 43036
Storage system, assembly	0477 42618
Shelf, movable	0477 43037
Retaining device	0477 40080
Cover freezing shelf	0477 43763
Specimen disc 37x37mm	0477 42603
Specimen disc 28x28mm	0477 42604
Bar, large	0477 42600
Bar, medium size	0477 42601
Bar, small	0477 42602
Heat extractor, Dr. Peters, assembly	0477 41338
Freezing griddle/block elevator	0201 39119
Dispensing slides 8 pieces	0201 39127
Foot rest assembly	0477 42832
Accessory kit extraction	0477 43300
Bactericidal filter 350/5865	0477 40296
Hose set, silicon 5 pieces	0477 44469
Filter assembly 25 pieces	0477 44307
Cryofect, VPE Leica Cryofect 4x 350ml,
low-temp. disinfectant to -20°C	0387 42801
Safety gloves size M	0340 29011
Safety gloves size S	0340 40859
Footswitch, dummy plug CM 3050	0443 30420
Laboratory chair on sliders (8030442)	0710 34911
Footswitch, assembly	0505 33888
Easy Dip staining container white	0712 40150
Easy Dip staining container pink	0712 40151
Easy Dip staining container green	0712 40152
Easy Dip staining container yellow	0712 40153
Easy Dip staining container blue	0712 40154
Easy Dip staining rack gray	0712 40161

54	Operating Manual V1.1 – 05/2007

Image 54

Leica CM1950 manual Cryofect, VPE Leica Cryofect 4x 350ml

Contents

Leica CM1950 Page Page Table of contents 12.2 11.112.1 12.3 Qualification of Personnel Symbols Used in This Manual and Their MeaningImportant Information Designated Use Safety and Design General Safety InstructionsUnpacking and Installation Opening the packaging Ramp assembly Transport to the Installation LocationRemoving the packaging Safety Features Microtome knives Knife guard Securing/Locking the Handwheel Cleaning, Disinfection Turning the Instrument Back On Maintenance Handling Specimens DefrostingRemoval of the Microtome Technical Data Technical Data Refrigeration system CM1950, 50 Hz CM1950, 60 Hz Standard Delivery Set of filters 5 pieces Handwheel, motorized General Overview Control Panel Fields and Cryostat Chamber Right side InstallationSite Requirements Left side Transport to the Site Assembling the Handwheel Locking/Unlocking the Handwheel Centering the Handwheel OptionalEmergency-stop function active, or Connection to Power Supply System Existing power systemOnly for instruments sold in Japan Selecting the frequency Installation Inserting the Section Waste Tray Installing the Storage Systems Optional For reasonsShelf, Movable Optional Section waste tray with the a Cutout a facing the user Installing the Heat Conductor Block, Stationary Optional Installation Mark for suction nozzle Instrument Controls Control Panel Fields on the CM1950 Control Panel Field 12 o’clock Position 6 o’clock 20.0μm Retraction0μm 60.0μm Coarse feed functions Motion continues as long as the button is heldBrief press of the button retracts the specimen by 20μm Control panel 3 Motorized sectioning optional Sectioning modes Disinfection Duration 30min Duration 180 min Leica Cryofect is not available in all countries Working with the Instrument Turning the Instrument On Configuring the Parameters Working with the Instrument Working with the Instrument Manual defrosting of the specimen cooling Entering the trimming thickness Working with the Precooled Cryostat 8.4.1 Preparatory Work Trimming with Extraction Anti-roll Guide Installed Handwheel position 12 3 o’clock, valve open all the way Cutting with Extraction Anti-roll Guide installed Shut off VAC LED in VAC button goes out Speed 10μm 40% 60% 80% 100% 20μm 30μm 40μmSpeed Extract. value 1μm 5 μm 40% 60% 80% 100% 10μm Working with the Instrument Troubleshooting Problems During WorkProblem Causes Remedies Cryochamber Sections tear Alternately Longer sufficient Temperature Selection Chart in minus C Tissue type Chamber temperature Optional Accessories Ordering Information Cryofect, VPE Leica Cryofect 4x 350ml Lever 6 for the lateral shift When using low-profile blades, the rulerWith anti-roll guide Lever 7 for clamping the blade Blade holder CE with finger rest for NB + BB Inserting the blade into knife holder CE Will result Cleaning the knife holder CE Daily cleaning Unscrew the bolts 35 of the pressure plate Not Visible Optional Accessories Anti-roll guide system Optional Accessories Specimen discs in various dimensions Optional Accessories Maintenance and Cleaning General Maintenance Changing Fuses Replacement of the Lamp/UVC Lamp Installation of the new lamp NB Microtome knives must be in boxes This equipment has been cleaned and decontaminatedDecontamination Certificate master Yes No indicated below BU Return Authorization Number Anne De Greef-Safft President Biosystems Division EC Declaration of Conformity Warranty and Service Peoples Republic of China