Leica CM1950 Manual - Comprehensive Guide to the CM1950 Projection Television

7.	Instrument Controls		30
	7.1	Control Panel Fields on the CM1950 – Control Panel Field 1	30
		Control Panel Field 2 – Electric Coarse Feed, Sectioning
		and Trimming Thickness Display	32
		Control panel 3 – Motorized sectioning (optional)	34

8.	Working with the Instrument		36
	8.1	Preparing Cutting Tools, Specimen Discs and Preparation Aids	36
	8.2	Turning the Instrument On	36
	8.3	Configuring the Parameters	37
	8.4	Working with the Precooled Cryostat	42
	8.4.1	Preparatory Work	42
	8.4.2	Trimming with Extraction – Anti-roll Guide Installed	43
	8.4.3	Cutting with Extraction – Anti-roll Guide Installed	45
9.	Troubleshooting		48
	9.1	Problems During Work	48
10.	Temperature Selection Chart (in minus °C)		52
11.	Optional Accessories		53
	11.1	Ordering Information	53
12.	Maintenance and Cleaning		66
	12.1	General Maintenance	66
	12.2	Changing Fuses	67
	12.3	Replacement of the Lamp/UVC Lamp	68
13.	Decontamination Certificate (master)		70
14.	EC Declaration of Conformity		72
15.	Warranty and Service		73
16.	Peoles Republic of China		74

Leica CM1950 – Cryostat

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Leica CM1950 manual 11.1, 12.1, 12.2, 12.3

Contents

Leica CM1950 Page Page Table of contents 12.1 11.112.2 12.3 Important Information Symbols Used in This Manual and Their MeaningQualification of Personnel Designated Use Unpacking and Installation Safety and DesignGeneral Safety Instructions Opening the packaging Removing the packaging Ramp assemblyTransport to the Installation Location Microtome knives Safety Features Securing/Locking the Handwheel Knife guard Cleaning, Disinfection Turning the Instrument Back On Removal of the Microtome MaintenanceHandling Specimens Defrosting Technical Data Refrigeration system CM1950, 50 Hz CM1950, 60 Hz Technical Data Set of filters 5 pieces Standard Delivery Handwheel, motorized General Overview Control Panel Fields and Cryostat Chamber Site Requirements InstallationRight side Left side Transport to the Site Assembling the Handwheel Emergency-stop function active, or Locking/Unlocking the HandwheelCentering the Handwheel Optional Only for instruments sold in Japan Selecting the frequency Connection to Power Supply SystemExisting power system Installation Shelf, Movable Optional Installing the Storage Systems Optional For reasonsInserting the Section Waste Tray Section waste tray with the a Cutout a facing the user Installing the Heat Conductor Block, Stationary Optional Installation Mark for suction nozzle Control Panel Fields on the CM1950 Control Panel Field Instrument Controls 12 o’clock Position 6 o’clock 0μm Retraction20.0μm 60.0μm Brief press of the button retracts the specimen by 20μm Coarse feed functionsMotion continues as long as the button is held Sectioning modes Control panel 3 Motorized sectioning optional Leica Cryofect is not available in all countries Disinfection Duration 30min Duration 180 min Turning the Instrument On Working with the Instrument Configuring the Parameters Working with the Instrument Working with the Instrument Manual defrosting of the specimen cooling Entering the trimming thickness Working with the Precooled Cryostat 8.4.1 Preparatory Work Trimming with Extraction Anti-roll Guide Installed Handwheel position 12 3 o’clock, valve open all the way Shut off VAC LED in VAC button goes out Cutting with Extraction Anti-roll Guide installed Speed Extract. value 1μm 5 μm 40% 60% 80% 100% 10μm Speed10μm 40% 60% 80% 100% 20μm 30μm 40μm Working with the Instrument Problem Causes Remedies TroubleshootingProblems During Work Cryochamber Sections tear Alternately Longer sufficient Tissue type Chamber temperature Temperature Selection Chart in minus C Ordering Information Optional Accessories Cryofect, VPE Leica Cryofect 4x 350ml With anti-roll guide When using low-profile blades, the rulerLever 6 for the lateral shift Lever 7 for clamping the blade Blade holder CE with finger rest for NB + BB Inserting the blade into knife holder CE Will result Unscrew the bolts 35 of the pressure plate Cleaning the knife holder CE Daily cleaning Not Visible Optional Accessories Anti-roll guide system Optional Accessories Specimen discs in various dimensions Optional Accessories General Maintenance Maintenance and Cleaning Changing Fuses Replacement of the Lamp/UVC Lamp Installation of the new lamp Decontamination Certificate master This equipment has been cleaned and decontaminatedNB Microtome knives must be in boxes Yes No indicated below BU Return Authorization Number EC Declaration of Conformity Anne De Greef-Safft President Biosystems Division Warranty and Service Peoples Republic of China