Leica CM1950 manual Working with the Instrument

8. Working with the Instrument

After trimming or sectioning:

Specimen:

•Unclamp and thaw.

•Immerse in fixative for further processing.

Cleaning

• Use the brush to sweep up the section waste (section waste tray) and remove it from the cryostat (follow applicable laboratory regulations for disposal).

Or:

•Clean the cryostat chamber using the chamber suction nozzle:

•To do so, turn the (flat) suction nozzle of the extraction hose by holding the hose on the white adapter and removing it with a quick twist. "Park" the flat suction nozzle in a designated place in the chamber, e.g. on the right inside wall of the cryogenic chamber.

•Remove the chamber suction nozzle from the plastic clip and attach it firmly to the white adapter.

•Check the remaining capacity of the filter (in the interior of the chamber) and change the filter if necessary (see page 28).

•Check the bacterial filter (in the front of the unit, see page 28), change it at least every 3 months.

•Move the anti-roll guide to the side.

•Spray the cryostat chamber using Cryofect.

•Activate the UVC disinfection.

Leica CM1950 Cryostat 47