Assembly/adjustment

Assembly

When taking the following steps, consult the manual for the Leica DM IRB/E manual.

!n. b.:

Before installing the LMC components, remove the field diaphragm. Also remove any filters, prisms and interference contrast components.

Screw the LMC objectives into the objective nosepiece.

Replace the condenser on the microscope with the S40/0.50 LMC condenser. First check that the inserted light slit diaphragms match the objectives in the nosepiece. The light slit dia- phragms are labelled, for example, LMC 10, LMC 20 or LMC 40.

Example: LMC 10 diaphragms belong to the C PLAN 10x/0.22 LMC objective.

The light diaphragms are usually assembled at the factory to match the supplied objectives. If the light slit diaphragms are supplied separately, they must be inserted in the positions in the condenser disc to match the objectives used.

Adjustment

Open the aperture diaphragm on the condenser fully.

Switch on the light. Select a medium brightness setting.

Set the condenser to the brightfield position and turn the first LMC objective into the light path (usually the objective with the smallest magnification).

Engage the Bertrand lens using the adjustment wheel on the right side of the microscope.

You will now see the modulator built into the objective as a grey rectangle at the edge of the field of view. Its position (top, bottom, left, right) is not fixed and may vary for different objectives. Focus the image of the modulator using the Bertrand lens.

Turn the condenser to switch to the light slit diaphragm whose name corresponds to the engraving on the objective (e. g. LMV 10 for the C PLAN 10x objective).

You will now see a bright rectangle.

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Leica DM IRB manual Assembly/adjustment, Screw the LMC objectives into the objective nosepiece, Adjustment