n. b.:

Long-term video microscopy

The solid and therefore stable basic body of the stand takes a while to stabilise thermally after the microscope is switched on. For investiga- tions taking over > 30 min. therefore, the microscope should be switched on about 1Ê – 2 hours beforehand.

Fig.Ê 80ÊLeicaÊ DM IRB, equipped with three TV cameras

Fig.Ê 81ÊSwitchÊ rods for beamsplitting

1 Upper beamsplitter switch rod (SIDE), 2 Lower beamsplitter switch rod (FRONT)

1

2

Fig.Ê 82ÊBeamsplittingÊ

1 100 % light to the tube, 2 80 % light to the side photo port, 20 % to the tube, 3 100 % light to the tube, 4 80 % light to the side photo port, 20 % to the tube, 5 50 % light to the front port, 50 % to the tube

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2

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

SIDE

 

 

 

 

 

 

 

SIDE

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

3

 

 

OFF

4

 

 

 

 

 

 

ON

5

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

SIDE

 

 

 

 

 

 

SIDE

 

 

 

 

SIDE

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

OFF

 

 

 

 

 

 

 

ON

 

 

 

 

 

OFF

 

 

 

FRONT

 

 

 

 

 

 

 

FRONT

 

 

 

 

 

FRONT

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

OFF

 

 

 

 

 

 

 

OFF

 

 

 

 

 

ON

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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Image 96
Leica manual Long-term video microscopy, Fig.Ê 80ÊLeicaÊ DM IRB, equipped with three TV cameras