Areas of application | Leica DM IRB specification

Areas of application

On the Leica DM IRB/E microscope, LMC is particularly suitable for life science applica- tions.

Use of birefingent materials

Transparent, living cultures in petri dishes can be observed in three dimensions, for example.

Use of a micromanipulator

The long free working distance of the S40/0.50 LMC condenser offers plenty of space for manipulation tools. The 3D image impression makes it easier to find suitable injection points.

Optical sectioning

LMC produces a large, flat observation area. This makes it easier to focus a specific area for observation.

Avoidance of halo effects

Phase contrast images are often spoiled by halo effects. These do not occur with LMC.

Use for fluorescing specimens

Morphology of fluorescing and non-fluorescing specimens can be analysed without changing the objective or moving the specimen.

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Image 102

Leica DM IRB manual Areas of application

Contents

Leica DM IRB Tel. +49 Leica DM IRB Copyrights Contents Operation Automatic lowering Text symbols and their meaning Important notes on this manual→ p General safety information Mains plug must only be inserted into a grounded outletPage Intended application Microscope and its components Key subassemblies Tube mount StandFirst of all, here is a general overview Stands Tube Condenser height adjustment Brightness adjustmentMains switch Coarse and fine control Objective nosepiece and objectives Specimen stages and accessoriesLamphousings IR/R tube adapter Installation site Ambient conditions Temperature 10 36 C Relative humidity Unpacking Installation Assembly Assembly toolsAssembly of the transmitted light illumination column Assembly of condensers Transmitted light illumination column, back view Condenser 0.30 S70 Position condenser disc, empty Assembly of IC condenser prisms Condenser 0.90 S1 bottom up Assembly of condenser 0.30 S70 Condenser top Assembly of condenser holder Condensers 0.53 S23 and 0.90 S1 Assembly of field diaphragm Assembly of filters and filter holder Retrofitting individual IC prisms Examples of prismsPrism a for objectives N Plan 5x Differences between prism D and D1 Inserting the analyserInserting the polariser Inserting the fluorescence module Fluorescence module Connect the lamp plug to the connecting socket in the stand Mirror housing and illumination telescope Lamphousing 107 L Assembling and exchanging incident light lamps Lamphousing 106 z L Lamphousing 106 z L Power units Type It is extremely important to heed the follow- ing advice Assembly of the tubes and tube adapter IR/R Bino HC BSA 25 Trino HC FSA 25 P and PR + PR = with and without back reflection Tube adapter R/IR HC, 2 Clamp screw Adapter, 6 Photo/TV port Transformer Edge of the mirror housing, 11 Mirror housing Inserting the eyepieces Inserting graticulesScrew the retainer ring back Inserting the photoeyepieces Screwing objectives in and outRemove the screw covers from the objective threads Plate x/y stage Only press the spring at the side Connecting the microscope to the mains Rotary stage and insert frame for coverslips E version DM IRB/E Features of the Leica DM IRB/E Important noteFunction and operation Assembly and initial installation Controls Learn mode Installing the objective prismsExit option in the display flashes Installing the objectives I T Display Phase contrast Display Objective magnificationDisplay IC objective prism Operating modes Dry/Immersion Immersion objectivesTo learn further objectives Dry objectives Exiting the Learn modeParfocality Oil immersion objectives Individual user adjustments Sw i t c he s Installing the fluorescence filter cube Concluding the installationWith the key, → b Motorized objective nosepiece DRY and Immersion Changing the operating modeOperating modes Now appears in the LC display at the bottom right Automatic lowering of the objective nosepiece Brightness adjustment Electronic focus Controls of the electronic focus are Coded IC objective prisms option Latter flashes if the combination is wrong Motorised fluorescence filter cube change option Connecting the control unit Control unit Keys on the control unit Component Name on the unit Lamp Focus Examples for the use of the footswitches Switch position Sensor OFF Switch position AutoSwitch position Light on Person sensor option 86mm S 10 0 xPH3 HH Front controls Operation Basic setting Switching on the halogen lamp Adjustment specimen Focusing the specimen Setting the tubes and eyepieces Operation of L objectives with correction mount Only when one eyepiece is without an adjustable eyelens Trinocular tube HCI 3T22 Side of the tube Operation of the side photo/TV port Operation of the front photo/TV port Colour coding of objectives Operation of objectivesImmersion objectives Locking objectivesPage Setting Koehler illumination Fig.Ê 62Ê Ê Possible errors Replace the eyepiece or disengage the Bertrand lens Operation of phase contrast Phase contrast observation Setting phase contrast with condensers 0.53 S23 and 0.90 S1 Operation of transmitted light darkfield Darkfield observation DL polarisation Crossing the polarisers Operation Transmitted light interference contrast TL interference contrast Centration of the condenser prisms Objectives for ICT Setting ICT contrast Sources of error if ICT image quality is unsatisfactoryChoice of prisms Operation of incident light fluorescence Fluorescence observation Alternative centration methods Method Centration with a centring aidMethod Centration in the rear focal plane of the objective Lamphousing 106 z L with halogen lamp, Xe and Hg lamps MethodCentration of 12 V 100 W halogen lamp Hg 50 W mercury lamp Hg 100 W and Xe 75 W lamps Halogen lamp Hg 50 lamp Xe 75 lamp Centring the aperture diaphragm Centring the field diaphragm Weak fluorescence, insufficient brightness Glass surfaces dirty Operation of filters Light filters Slides with the following line patterns are available Operation of the slide overlay deviceSlide overlay device Scale 10 mm = 100 divisions Operation of the macro device Fig.Ê 73ÊMacroÊ device on FSA 25 PE tube with tube adapter Field of view Ø 10x/20 eyepiece Total magnification in the eyepiece would therefore be Transmitted light stage micrometer for cali- bration Length measurementsFollowing components are required for length measurements Calibration With zoom magnification Vario TV adapter MTV = objective magnification x tube factor Monitor diameter Long-term video microscopy Fig.Ê 80ÊLeicaÊ DM IRB, equipped with three TV cameras Operation of LMC Principle of LMC Principle LMC consists of the following components ComponentsComponents S40/0.50 LMC condenser Adjustment Assembly/adjustmentAssembly Screw the LMC objectives into the objective nosepiecePage Areas of application Care and maintenance Cleaning objectives Acids, alkaline solutionsRemoval of immersion oil Electric errors TroubleshootingMechanical errors On/off switch does not respond no illumination Additional fluorescence lamp does not respond 106 Schutter FST 846-205.000-00Name 107 Ê Lamphousing 106 z, opened 108 Replacing the 12 V 100 W halogen lamp in lamphousing 106 z 109 Changing the Hg and Xe lamps on lamphousing 106 z 111 Storage Transport Objective lettering Technical descriptionPerformance data of objectives 0Ê -Ê 10x/0.22 Or A, B, CObjective type performance class Plan 114 Colour coding of the objectives White WaterLocking of objectives Performance data of eyepieces Decisive value is always the smallestEyepiece field of view Field performance of objectives Eyepiece graticules BG20 Filter performance data Use Grey/neutral density filter NGreen filter, GR, panchromatic CB1.5, CB3 Binocular ergotube HCI BV22 Tube performance dataBinocular tube HCI B22 Like HCI B22, but with variable viewing angle of 15Ê -Ê Trinocular tube HCI 3T22 Tube adapter R/IR Condenser performance data Condenser 0.30 S70 121 Condenser disc Object guide122 Lamphousing 107/2 Lamphousing performance dataLamphousing 106 z Stages Plane stage and mountable object guide Xe high pressure lamp 75 W D.C., stabilised TypeHg ultra high pressure lamp 50 W A.C C., stabilised/non-stabilised Fuses Ambient temperature 10Ê -Ê 36C Relative humidity General technical dataGeneral technical data Overvoltage category Contamination class 125 Technical data of the power unit General technical data Technical specificationsULa = lamp voltage Main wearing and spare parts, tools 127 EU Conformity declaration Product namePage Ernst-Leitz-Straße