Leica DM IRB manual Centration of the condenser prisms, Objectives for ICT

Centration of the condenser prisms

If you have ordered a complete microscope, this adjustment will already have been made at the factory. However, it is advisable to check the centration from time to time, particularly after transport: disengage the objective-side IC prisms (pos. H).

Remove an eyepiece from the eyepiece tube. Engage the condenser-side IC prisms one after the other (the whole-wave compensator must not be active, i. e. the lambda engraving is on the bottom side of the analyser). When the cen- tration is correct, the dark stripe must be in the centre of the pupil (= brighter circle) of the objective (Fig. 65).

If not, proceed as follows:

Put one of the supplied centring keys in the disc opening on the left of the label plate for the IC prism (e. g. 64.2) (Fig. 65) and turn it to centre the stripe.

Objectives for ICT

Transmitted light interference contrast is possible with the brightfield and phase contrast objectives which have the code letter of the pupil position in the first line of engraving, e. g. A (see separate objective chart).

An IC condenser prism, e. g. K6, must also be available for the objective. An up-to-date table of possible prism combinations (objective chart) is enclosed separately with each configuration.

Fig.Ê 65	Fig.Ê 66Centration for interference contrast
Objective pupil with correctly centred compensation stripe	1 Centring keys in working position

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