Leica DM IRB manual Operation of incident light fluorescence, Fluorescence observation

Operation of incident light fluorescence

n. b.:

Only with microscope with integrated incident light fluorescence axis.

Fluorescence observation

Focus the specimen in transmitted light first, if possible (perhaps Phaco or ICT).

Select a filter cube to suit the excitation and emission spectrum of the specimen and move into the light path by rotating the turret. Open the iris diaphragm of the objective.

Switch the magnification changer* (on the DM IRB-SLR and DM IRBE versions) by turning the knurl to pos. 1x. Switch off the transmitted light illumination.

Open the incident light path. The switch rod should be pulled out fully.

If the background is too red, engage a BG 38 filter. Push the switch rod in halfway.

The BG 38 filter should always be used for photography.

When not looking through the microscope, always block the incident light path to prevent specimens fading. Push the switch rod in all the way.

The 3 clickstop positions of the switch rod mean:

Switch rod
Stop ⬤	Incident light path blocked
	(light stop)
BG 38	BG filter engaged
◯	Incident light path open

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