Official Manual for Leica DM IRB Microscope & Magnifier

General technical data

General technical data
For indoor use only
Mains voltage:	90Ê –Ê 250 V ~
Frequency:	50Ê –Ê 60 Hz
Power consumption:	DM IRB max. 160 W
Fuses:	T 4 A
Ambient temperature:	10Ê –Ê 36°C
Relative humidity:	0Ê –Ê 80 % up to 30°C
Overvoltage category:	II
Contamination class:	2

125

Image 125

Leica DM IRB manual General technical data, Fuses Ambient temperature 10Ê -Ê 36C Relative humidity

Contents

Leica DM IRB Tel. +49 Leica DM IRB Copyrights Contents Automatic lowering Operation → p Text symbols and their meaningImportant notes on this manual Mains plug must only be inserted into a grounded outlet General safety informationPage Intended application Key subassemblies Microscope and its components First of all, here is a general overview Stands StandTube mount Tube Mains switch Brightness adjustmentCondenser height adjustment Coarse and fine control Lamphousings Specimen stages and accessoriesObjective nosepiece and objectives IR/R tube adapter Ambient conditions Temperature 10 36 C Relative humidity Installation site Unpacking Installation Assembly of the transmitted light illumination column AssemblyAssembly tools Transmitted light illumination column, back view Assembly of condensers Condenser 0.30 S70 Position condenser disc, empty Condenser 0.90 S1 bottom up Assembly of IC condenser prisms Condenser top Assembly of condenser 0.30 S70 Condensers 0.53 S23 and 0.90 S1 Assembly of condenser holder Assembly of filters and filter holder Assembly of field diaphragm Prism a for objectives N Plan 5x Retrofitting individual IC prismsExamples of prisms Inserting the polariser Differences between prism D and D1Inserting the analyser Fluorescence module Inserting the fluorescence module Mirror housing and illumination telescope Connect the lamp plug to the connecting socket in the stand Assembling and exchanging incident light lamps Lamphousing 107 L Lamphousing 106 z L Lamphousing 106 z L Type Power units It is extremely important to heed the follow- ing advice Assembly of the tubes and tube adapter IR/R + PR = with and without back reflection Bino HC BSA 25 Trino HC FSA 25 P and PR Tube adapter R/IR HC, 2 Clamp screw Adapter, 6 Photo/TV port Transformer Edge of the mirror housing, 11 Mirror housing Screw the retainer ring back Inserting the eyepiecesInserting graticules Remove the screw covers from the objective threads Inserting the photoeyepiecesScrewing objectives in and out Only press the spring at the side Plate x/y stage Rotary stage and insert frame for coverslips Connecting the microscope to the mains Function and operation E version DM IRB/E Features of the Leica DM IRB/EImportant note Controls Assembly and initial installation Exit option in the display flashes Learn modeInstalling the objective prisms I T Installing the objectives Display IC objective prism Display Phase contrastDisplay Objective magnification To learn further objectives Operating modes Dry/ImmersionImmersion objectives Parfocality Exiting the Learn modeDry objectives Oil immersion objectives Sw i t c he s Individual user adjustments With the key, → b Installing the fluorescence filter cubeConcluding the installation Motorized objective nosepiece Operating modes Changing the operating modeDRY and Immersion Now appears in the LC display at the bottom right Automatic lowering of the objective nosepiece Brightness adjustment Controls of the electronic focus are Electronic focus Latter flashes if the combination is wrong Coded IC objective prisms option Motorised fluorescence filter cube change option Control unit Connecting the control unit Component Name on the unit Keys on the control unit Lamp Focus Examples for the use of the footswitches Switch position Light on Switch position AutoSwitch position Sensor OFF Person sensor option Front controls 86mm S 10 0 xPH3 HH Switching on the halogen lamp Operation Basic setting Focusing the specimen Adjustment specimen Operation of L objectives with correction mount Setting the tubes and eyepieces Only when one eyepiece is without an adjustable eyelens Side of the tube Trinocular tube HCI 3T22 Operation of the front photo/TV port Operation of the side photo/TV port Immersion objectives Operation of objectivesColour coding of objectives Locking objectivesPage Fig.Ê 62Ê Ê Setting Koehler illumination Replace the eyepiece or disengage the Bertrand lens Possible errors Phase contrast observation Operation of phase contrast Setting phase contrast with condensers 0.53 S23 and 0.90 S1 Darkfield observation Operation of transmitted light darkfield Crossing the polarisers DL polarisation TL interference contrast Operation Transmitted light interference contrast Objectives for ICT Centration of the condenser prisms Choice of prisms Setting ICT contrastSources of error if ICT image quality is unsatisfactory Fluorescence observation Operation of incident light fluorescence Method Centration in the rear focal plane of the objective Alternative centration methodsMethod Centration with a centring aid Centration of 12 V 100 W halogen lamp Lamphousing 106 z L with halogen lamp, Xe and Hg lampsMethod Hg 100 W and Xe 75 W lamps Hg 50 W mercury lamp Halogen lamp Hg 50 lamp Xe 75 lamp Centring the field diaphragm Centring the aperture diaphragm Glass surfaces dirty Weak fluorescence, insufficient brightness Light filters Operation of filters Slide overlay device Operation of the slide overlay deviceSlides with the following line patterns are available Scale 10 mm = 100 divisions Fig.Ê 73ÊMacroÊ device on FSA 25 PE tube with tube adapter Operation of the macro device Field of view Ø 10x/20 eyepiece Total magnification in the eyepiece would therefore be Following components are required for length measurements Length measurementsTransmitted light stage micrometer for cali- bration Calibration With zoom magnification Vario TV adapter MTV = objective magnification x tube factor Monitor diameter Fig.Ê 80ÊLeicaÊ DM IRB, equipped with three TV cameras Long-term video microscopy Operation of LMC Principle Principle of LMC Components ComponentsLMC consists of the following components S40/0.50 LMC condenser Assembly Assembly/adjustmentAdjustment Screw the LMC objectives into the objective nosepiecePage Areas of application Care and maintenance Removal of immersion oil Cleaning objectivesAcids, alkaline solutions Mechanical errors TroubleshootingElectric errors On/off switch does not respond no illumination 106 Additional fluorescence lamp does not respond Name 846-205.000-00Schutter FST 107 108 Ê Lamphousing 106 z, opened 109 Replacing the 12 V 100 W halogen lamp in lamphousing 106 z Changing the Hg and Xe lamps on lamphousing 106 z 111 Transport Storage Performance data of objectives Technical descriptionObjective lettering 0Ê -Ê Objective type performance class Plan Or A, B, C10x/0.22 114 Locking of objectives Colour coding of the objectivesWhite Water Eyepiece field of view Performance data of eyepiecesDecisive value is always the smallest Eyepiece graticules Field performance of objectives Green filter, GR, panchromatic Filter performance data Use Grey/neutral density filter NBG20 CB1.5, CB3 Binocular tube HCI B22 Tube performance dataBinocular ergotube HCI BV22 Like HCI B22, but with variable viewing angle of 15Ê -Ê Tube adapter R/IR Trinocular tube HCI 3T22 121 Condenser performance data Condenser 0.30 S70 122 Condenser discObject guide Lamphousing 106 z Lamphousing performance dataLamphousing 107/2 Stages Plane stage and mountable object guide Hg ultra high pressure lamp 50 W A.C TypeXe high pressure lamp 75 W D.C., stabilised C., stabilised/non-stabilised General technical data General technical dataFuses Ambient temperature 10Ê -Ê 36C Relative humidity Overvoltage category Contamination class 125 ULa = lamp voltage Technical data of the power unit General technical dataTechnical specifications 127 Main wearing and spare parts, tools Product name EU Conformity declarationPage Ernst-Leitz-Straße