Leica DM IRB manual Operation of LMC

Operation of LMC

Leica modulation contrast (LMC) is a special form of oblique illumination based on the principle of Hoffmann modulation contrast.

In this technique, the phase gradients of an unstained specimen are converted into dif- ferences in amplitude with the aid of a modu- lator.

This gives a three-dimensional impression simi- lar to an interference contrast image. Unlike interference contrast, however, the specimen can be observed through birefringent plastic materials such as petri dishes.

Further advantages of this technique are:

–high contrast

–high resolution

–halo-free, high-contrast relief image

–long free working distance of the condenser

–easy assembly and adjustment

–use for both stained and unstained specimens.

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