Leica DM IRB manual

The light slit diaphragm is now adjusted until the bright stripe of the slit image is fully inside the grey stripe of the modulator. The light slit diaphragm can be rotated and moved in x and y direction.

For the 10x objective the image of the modulator and the light slit are virtually the same size. Adjust the light slit diaphragm until the bright slit lies near the dark edge.

Repeat this process for each objective.

Always make sure that the objective name and the name of the light slit diaphragm coincide.

Then disengage the Bertrand lens with the adjustment wheel on the right side of the microscope. Switch on magnification 1x or higher and put a specimen on the stage.

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