Leica DM IRB manual Adjustment specimen, Focusing the specimen

Adjustment specimen

For initial microscope adjustment we recom- mend you use a specimen that has both high and low contrast areas.

It is easier to focus incident light fluorescence specimens in transmitted light first.

Focusing the specimen

(For the DM IRB version, please read the section about the operation of the E focus and objective nosepiece first. Here, an example of manual operation is given for each case.)

Focus the specimen you want to examine. To do this, the objective nosepiece should be lowered first. The objective is turned into the light path by rotating the black knurled knob on the nose- piece. The objective should click audibly into position.

Focus the specimen with the coarse and fine drive, which changes the height of the objec- tive nosepiece. The stage height remains un- changed. The total vertical travel of the nosepiece is 7 mm. In air, the focusing range extends from 2 mm below the stage surface to 5 mm above it.

One drum interval of the fine focusing cor- responds to about 2 mm of the objective nose- piece.

!Caution!

Please be careful with high objective magni- fications when focusing or making x-y adjust- ments!

When using objectives with a high magnification and a short working distance (from 50x), the specimen and the stage insert may be lifted and tilted.

When scanning the specimen, the front lens of the objective may knock against the edge of the stage insert.

Lower the coarse and fine drive if possible when turning the nosepiece and changing the ob- jectives, in order to avoid contact between the front lens and the stage insert.

!n. b.

Caution with special objectives! Here there may be contact between the stage insert and the front lens the moment the objective is moved over the edge of the inner hole of the stage insert!

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