Leica DM IRB manual Condenser disc, Object guide, 122

Together with condenser top 0.53 S23, max. FWD 30 mm, culture vessels can be examined microscopically up to liquid levels of 25 mm. For contrasting techniques brightfield (HF), phase contrast (Phaco/PH), transmitted light inter- ference contrast (ICT) and polarisation contrast, objectives with magnifications up to 100x can be used. For transmitted light darkfield we recommend objectives with a numerical aper- ture up to 0.40.

For condenser top 0. 90 S1, max. FWD 1 mm, thin specimen slides and coverslips must be used as substrates for the specimen. Objectives with a numerical aperture up to 0.75 are suitable for transmitted light darkfield. All other contrasting techniques can be performed up to objective magnification 100x.

Condenser disc

All condensers of the Leica DM IRB are equipped with a 6-position disc which can be fitted with an individual choice of annular stops for phase contrast (PH), darkfield (DF) or IC prisms for transmitted light interference con- trast (ICT).

Performance data of stages and mountable object guides

Plane stage

With hole for insert rings of 20 mm diameter or 40 mm diameter. Holes for inserting specimen clips and two threaded holes on left and right underneath the stage for attachment of the object guide.

Object guide

Adjustment range: X 127 mm x Y 83 mm

To accommodate holders for different culture vessels. Self-adhesive scales for the holders are enclosed for coordinate adjustment reading. These should be stuck in the countersinks of the object guide.

3-plate x/y stage

Adjustment range: X 60 mm x Y 40 mm

With hole for insert rings of 20 mm diameter or 40 mm diameter. Holes for inserting specimen clips. Coaxial drive for specimen positioning with universal joint.

Rotary stage with object guide and frame insert for coverslips

Rotation radius: 360°

Adjustment range of the object guide: X 40 mm x Y 40 mm

The object guide accommodates specimen slides or the frame insert for coverslips. The minimum size of coverslips that can be secured in the frame insert is 22 mm x 32 mm.

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