Leica DM IRB Setting the tubes and eyepieces, Operation of L objectives with correction mount

Checking of various microscope components

Engage or disengage the filters (54.16) accord- ing to the required brightness.

If necessary, disengage the Bertrand lens by turning the knurled knob (54.22), pos. 1. Disengage the analyser (55.21), if necessary, by pulling it out partway.

Disengage the filter systems, if necessary, by rotating the turret (55.11).

Push in the switch rod(s) for the beamsplitter (54.23).

Clamp the transmitted light illumination arm with the knurled wheel (5.1).

Fig. 56Ê Ê Examples of objectives

1, 2 Objectives with correction mounts (Corr) for adjusting to different vessel base thicknesses (e. g. 0.1 – 1.3 mm and 0 – 2 mm), 3 Objective with built-in iris diaphragm (1.30 = maximum aperture, 0.60 = minimum aperture), 4 Magnifica- tion colour code, 5 Knurled ring for adjusting the correction mount, 6 Knurled ring for adjusting the built-in diaphragm

Operation of L objectives with correction mount

Roughly set the correction mount to the thick- ness of the base of the vessel on the stage by turning the knurled ring. Focus the specimen with the coarse and fine drive. Then operate the correction mount until you achieve the greatest image contrast, using the fine focus if neces- sary.

Setting the tubes and eyepieces

Eyeglass wearers must remove (for 10x/25) or push back (for 10x/20 and 10x/22) the anti-glare protection of the eyepieces, but it should always be left on for viewers not wearing eyeglasses.

●Set the interpupillary distance on the tube by pulling apart or pushing together the eyepiece tubes until only one image can be seen with both eyes.

●Note your personal interpupillary distance.

●If using ergotubes, set the viewing angle (15° – 50°) as well by tilting the binocular

viewing port. To avoid symptoms of fatigue, vary the viewing angle from time to time.

●Close any tube exits you are not using to prevent stray light disturbing the image.

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