Leica DM IRB manual Operation Transmitted light interference contrast, TL interference contrast

Operation

of transmitted light interference contrast

TL interference contrast

TL interference contrast observation is possible with condenser 0.30 S70 with objective mag- nifications from 10x to 40x, with condensers

0.53S23 or 0.90 S1 from 10x to 100x. For objective 100x there is also a condenser top P 1.40 OIL S1 for extremely high resolution.

Crossing the polarisers

Remove the Bertrand lens and fluorescence filter cube from the light path if necessary; turn the condenser disc and turret for objective-side IC prisms to pos. H. Focus the specimen (20x objective). Set Koehler illumination exactly (not needed for condenser 0.30 S70). Remove the specimen from the light path.

Insert a polariser into the filter holder with the engraving facing upwards.

Turn the filter holder to the right into the light path.

Push the analyser into the 2nd clickstop position in the microscope with the engraving ICT facing upwards.

Set the optimum extinction position by rotating the polariser and watching the empty field of view.

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