Copyrights | Leica DM IRB guide

Copyrights

All rights to this documentation and the software it describes are owned by Leica Microsystems Wetzlar GmbH. Copying of text and illustrations – in full or in part – by printing, photostat, microfilm or other techniques, in- cluding electronic systems, is only permitted subject to the express written consent of Leica Microsystems Wetzlar GmbH.

The information contained in the following documentation represents the latest stage of technology and knowledge. We have composed the texts and illustrations with great care. However, as it is impossible to eliminate the risk of error completely, we cannot accept any kind of liability for the correctness of the contents of this manual. Nevertheless, we are always grateful to be notified of any errors.

The information in this manual may be altered without prior notice.

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Leica DM IRB manual Copyrights

Contents

Leica DM IRB Tel. +49 Leica DM IRB Copyrights Contents Operation Automatic lowering Important notes on this manual Text symbols and their meaning→ p General safety information Mains plug must only be inserted into a grounded outletPage Intended application Microscope and its components Key subassemblies Stand First of all, here is a general overview StandsTube mount Tube Brightness adjustment Mains switchCondenser height adjustment Coarse and fine control Specimen stages and accessories LamphousingsObjective nosepiece and objectives IR/R tube adapter Installation site Ambient conditions Temperature 10 36 C Relative humidity Unpacking Installation Assembly tools AssemblyAssembly of the transmitted light illumination column Assembly of condensers Transmitted light illumination column, back view Condenser 0.30 S70 Position condenser disc, empty Assembly of IC condenser prisms Condenser 0.90 S1 bottom up Assembly of condenser 0.30 S70 Condenser top Assembly of condenser holder Condensers 0.53 S23 and 0.90 S1 Assembly of field diaphragm Assembly of filters and filter holder Examples of prisms Retrofitting individual IC prismsPrism a for objectives N Plan 5x Inserting the analyser Differences between prism D and D1Inserting the polariser Inserting the fluorescence module Fluorescence module Connect the lamp plug to the connecting socket in the stand Mirror housing and illumination telescope Lamphousing 107 L Assembling and exchanging incident light lamps Lamphousing 106 z L Lamphousing 106 z L Power units Type It is extremely important to heed the follow- ing advice Assembly of the tubes and tube adapter IR/R Bino HC BSA 25 Trino HC FSA 25 P and PR + PR = with and without back reflection Tube adapter R/IR HC, 2 Clamp screw Adapter, 6 Photo/TV port Transformer Edge of the mirror housing, 11 Mirror housing Inserting graticules Inserting the eyepiecesScrew the retainer ring back Screwing objectives in and out Inserting the photoeyepiecesRemove the screw covers from the objective threads Plate x/y stage Only press the spring at the side Connecting the microscope to the mains Rotary stage and insert frame for coverslips Important note E version DM IRB/E Features of the Leica DM IRB/EFunction and operation Assembly and initial installation Controls Installing the objective prisms Learn modeExit option in the display flashes Installing the objectives I T Display Objective magnification Display Phase contrastDisplay IC objective prism Immersion objectives Operating modes Dry/ImmersionTo learn further objectives Exiting the Learn mode ParfocalityDry objectives Oil immersion objectives Individual user adjustments Sw i t c he s Concluding the installation Installing the fluorescence filter cubeWith the key, → b Motorized objective nosepiece Changing the operating mode Operating modesDRY and Immersion Now appears in the LC display at the bottom right Automatic lowering of the objective nosepiece Brightness adjustment Electronic focus Controls of the electronic focus are Coded IC objective prisms option Latter flashes if the combination is wrong Motorised fluorescence filter cube change option Connecting the control unit Control unit Keys on the control unit Component Name on the unit Lamp Focus Examples for the use of the footswitches Switch position Auto Switch position Light onSwitch position Sensor OFF Person sensor option 86mm S 10 0 xPH3 HH Front controls Operation Basic setting Switching on the halogen lamp Adjustment specimen Focusing the specimen Setting the tubes and eyepieces Operation of L objectives with correction mount Only when one eyepiece is without an adjustable eyelens Trinocular tube HCI 3T22 Side of the tube Operation of the side photo/TV port Operation of the front photo/TV port Operation of objectives Immersion objectivesColour coding of objectives Locking objectivesPage Setting Koehler illumination Fig.Ê 62Ê Ê Possible errors Replace the eyepiece or disengage the Bertrand lens Operation of phase contrast Phase contrast observation Setting phase contrast with condensers 0.53 S23 and 0.90 S1 Operation of transmitted light darkfield Darkfield observation DL polarisation Crossing the polarisers Operation Transmitted light interference contrast TL interference contrast Centration of the condenser prisms Objectives for ICT Sources of error if ICT image quality is unsatisfactory Setting ICT contrastChoice of prisms Operation of incident light fluorescence Fluorescence observation Method Centration with a centring aid Alternative centration methodsMethod Centration in the rear focal plane of the objective Method Lamphousing 106 z L with halogen lamp, Xe and Hg lampsCentration of 12 V 100 W halogen lamp Hg 50 W mercury lamp Hg 100 W and Xe 75 W lamps Halogen lamp Hg 50 lamp Xe 75 lamp Centring the aperture diaphragm Centring the field diaphragm Weak fluorescence, insufficient brightness Glass surfaces dirty Operation of filters Light filters Operation of the slide overlay device Slide overlay deviceSlides with the following line patterns are available Scale 10 mm = 100 divisions Operation of the macro device Fig.Ê 73ÊMacroÊ device on FSA 25 PE tube with tube adapter Field of view Ø 10x/20 eyepiece Total magnification in the eyepiece would therefore be Length measurements Following components are required for length measurementsTransmitted light stage micrometer for cali- bration Calibration With zoom magnification Vario TV adapter MTV = objective magnification x tube factor Monitor diameter Long-term video microscopy Fig.Ê 80ÊLeicaÊ DM IRB, equipped with three TV cameras Operation of LMC Principle of LMC Principle Components ComponentsLMC consists of the following components S40/0.50 LMC condenser Assembly/adjustment AssemblyAdjustment Screw the LMC objectives into the objective nosepiecePage Areas of application Care and maintenance Acids, alkaline solutions Cleaning objectivesRemoval of immersion oil Troubleshooting Mechanical errorsElectric errors On/off switch does not respond no illumination Additional fluorescence lamp does not respond 106 846-205.000-00 NameSchutter FST 107 Ê Lamphousing 106 z, opened 108 Replacing the 12 V 100 W halogen lamp in lamphousing 106 z 109 Changing the Hg and Xe lamps on lamphousing 106 z 111 Storage Transport Technical description Performance data of objectivesObjective lettering 0Ê -Ê Or A, B, C Objective type performance class Plan10x/0.22 114 White Water Colour coding of the objectivesLocking of objectives Decisive value is always the smallest Performance data of eyepiecesEyepiece field of view Field performance of objectives Eyepiece graticules Filter performance data Use Grey/neutral density filter N Green filter, GR, panchromaticBG20 CB1.5, CB3 Tube performance data Binocular tube HCI B22Binocular ergotube HCI BV22 Like HCI B22, but with variable viewing angle of 15Ê -Ê Trinocular tube HCI 3T22 Tube adapter R/IR Condenser performance data Condenser 0.30 S70 121 Object guide Condenser disc122 Lamphousing performance data Lamphousing 106 zLamphousing 107/2 Stages Plane stage and mountable object guide Type Hg ultra high pressure lamp 50 W A.CXe high pressure lamp 75 W D.C., stabilised C., stabilised/non-stabilised General technical data General technical dataFuses Ambient temperature 10Ê -Ê 36C Relative humidity Overvoltage category Contamination class 125 Technical specifications Technical data of the power unit General technical dataULa = lamp voltage Main wearing and spare parts, tools 127 EU Conformity declaration Product namePage Ernst-Leitz-Straße