Leica DM IRB manual DL polarisation, Crossing the polarisers

Operation of transmitted light polarisation

DL polarisation

Polarisation contrast for examining birefringent specimens is possible with condenser 0.30 S70 with objective magnifications from 2.5x to 40x, with condensers 0.53 S23 or 0.90 S1 from 5x or 10x to 100x. A P 1.40 OIL S1 condenser top is also available for extremely hgh resolution.

Crossing the polarisers

First: Set Koehler illumination. Remove the speci- men from the light path; remove the Bertrand lens and fluorescence filter cube if necessary; turn the condenser disc and turret for objective- side IC prisms to pos. H.

Insert the polariser into the filter holder with the engraving facing upwards. Turn the filter holder to the right into the light path.

Push the analyser into the 2nd clickstop position in the microscope with the engraving ICT facing upwards.

Set the optimum extinction position by rotating the polariser and watching the empty field of view. Put a specimen on the stage.

For Pol colour contrast, the ICT analyser can be turned over, with the lambda engraving facing upwards, to activate a whole-wave compensator.

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