Teledyne 10 manual Operation, Sample Preparation Column Installation, Adapters

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BioOptix™ 10

Section 3 Operation

3.1Sample Preparation

3.2Column Installation

This section provides an introduction to preparing the system and creating files necessary for a purification run. The instruc- tions assume that the system has been set up according to Section 2.

The Sample In lines for each channel should be placed into a vessel holding the liquid sample mixture. Sample In lines can be placed into a common sample vessel, or in multiple vessels when unique samples are to be introduced on each channel.

The BioOptix 10 system uses versatile column clamps to hold a variety of columns. The clamps are adjustable, holding column sizes as large as 45 mm (13/4 in).

Note

User-supplied clamps and apparatus may be used on the sys- tem. The mounting holes on the side of the BioOptix 10 accept 9.5 mm (3/8 in) mounting stems.

To install a column, insert the column into the clamp and turn the knob to close the clamp and secure the column.

When inserting any column be sure to observe the flow direction if noted on the column. Orient the column with the inlet at the top and the outlet at the bottom.

3.2.1Column Tubing and Because of the variety of columns that may be used on the

Adapters	BioOptix 10, the system does not include standard fittings. All
	column fittings and tubing are user-supplied to fit the selected
	columns.
	To install the column tubing:

1. Remove the bypass tubing that was installed to prime/purge the pumps.

2. At each column position, insert a tube with a fitting at the upper column inlet port. Then, connect the fitting to the top of the column.

3. At each column position, insert a tube with a fitting at the lower column outlet port. Then, connect the fitting to the bottom of the column.

3-1

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Contents BioOptix Page Foreword Page Application, take heed in the following general warnings General WarningsDamage Symboles de sécurité Hazard SymbolsBioOptix 10 Safety BioOptix Table of ContentsAppendix B Fluid Path Diagrams System MaintenanceAppendix C Replacement Parts Lists Appendix a Method TemplatesList of Figures List of Tables Product Overview IntroductionMaximum Altitude 2000 meters BioOptix 10 SpecificationsBioOptix 10 Front Panel Controls System Controls and ConnectorsBioOptix 10 Front Panel Controls BioOptix 10 Left-side Controls and Connections BioOptix 10 Left-side ConnectionsBioOptix 10 Right-side Connections BioOptix 10 Right-side ConnectionsBioOptix 10 Rear Panel Controls and Connections BioOptix 10 Rear Panel Controls and ConnectionsBioOptix 10 Installation InstallationUnpacking the BioOptix 10 System SpaceAssembling ElectricalBioOptix Accessing pump and column module internal connections Routing the Sample Out lines column module P110 Connection on BD3 AC power connection from pump module 10 Liquid connections pump module shown 11 Aligning the fasteners Foxy Ceeding with the arm assembly instructionsBioOptix Installation Common Buffer Inlet Mounting Block Manifold Channel Numbers Color Part Number Tubing Color chart16 Foxy 200 tubing connections Windows PC Minimum Requirements ConfiguringBioOptix 10 Software BioOptix 10 System Preparation 18 Column bypass tubing 20 BioOptix 10 Manual Hardware Control window BioOptix Installation Operation Sample Preparation Column InstallationAdapters Gradients BioOptix 10 SoftwareOverview Defining a Gradient To define a gradient MethodsEquilibrate tab settings Load tab settings Load Settings This block loads the sample onto the columnWash Settings Experiments Flush SettingsSetting up Experiment RunningOperation Buttons FileOperation Zoom and panPump Flush Post-experiment OperationPreview Saving Run DataAutoReports BioOptix 10 Operation Vent damage System MaintenanceExterior Cleaning Piston Seal Replacement Refer to -3 for the following steps Pump assembly components Deuterium Lamp Replacement Waste port mounting plate Common Problems TroubleshootingOperating Problems Inaccessible Fuses Circuit breaker location How to Ship Returns Service DepartmentTeledyne Isco, Inc Appendix a Method Templates Dard.tpl General Editing Rules Modifying TemplateBlockn BlocksOther Fluid-type Blocks Appendix B Fluid Path DiagramsLoad-type Blocks BioOptix Appendix B Fluid Path DiagramsFigure B-4 Fluid path for Load-type blocks Manual injections Pump Flush Plumbing DiagramFigure B-7 BioOptix 10 Plumbing Diagram BioOptix Appendix B Fluid Path Diagrams Appendix C Replacement Parts Lists BioOptix Appendix C Replacement Parts Lists Detector Assembly 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists Column Module 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists Pump Module 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists Declaration of Conformity Page Teledyne Isco One Year Limited Factory Service Warranty