Teledyne 10 manual Load-type Blocks, BioOptix Appendix B Fluid Path Diagrams

BioOptix™ 10

Appendix B Fluid Path Diagrams

While the system is running a fluid-type block:

•The Buffer valve switches between buffers A and B to produce the correct mix.

•The Sample valve allows only buffer solution to pass.

•If the channel is active, the Channel valve allows the fluid to be pumped. If the channel is inactive, the valve prevents fluid from being pumped (Figure B-2).

•The Mode valve is in the Operate position.

•The Effluent valve can direct the fluid to waste or to the fraction collector. Directing the effluent to waste is practical when the fluid block is in the experiment for column equilibration. Collecting the effluent may be desirable if the fluid may contain something of interest (e.g. during a column wash).

Channel

Column

Pump

Figure B-2 Inactive channel

B.2 Load-type Blocks

Load blocks apply the sample onto the column. There are two modes that can be selected in the Method file—Pump or Manual injection.

Pump injections draw liquid sample from the Sample In port and routes it to the column (Figure B-3). The Mode valve should be in the Operate position; the Effluent valve can either collect the fluid or divert it to the channel’s Waste port.

ChannelMode

Sample

Sample In

Column

Pump

Effluent

To Foxy

Figure B-3 Fluid path for Load-type blocks -

Pump injections

B-2