Teledyne 10 manual Zoom and pan, Operation

BioOptix™ 10

Section 3 Operation

Figure 3-9 Pause at end of block

If the block is set to collect effluent (Figure 3-4), the fraction col- lector will deposit the fluid in the collection tubes. If a peak is detected, the fraction collector automatically advances to the next row to isolate the fluid. The fraction collector will also auto- matically advance to the next row of tubes whenever the spec- ified fraction size has been collected.

If the block is not set to collect effluent (Figure 3-2), the

BioOptix 10 diverts all pumped fluids to the waste ports.

3.7.2Operation Displays The window displayed during the run has several features that let you monitor the operation.

	At the start of a run, the plot area displays the gradient as a blue
	line (right Y-axis). The X-axis displays the volume pumped.
	Below the X-axis is a progress bar. As the run progresses, the
	absorbance traces is plotted as a red line (left Y-axis).
Zoom and pan	To the right of the plot area are several controls that can modify
	the display. The Pan/Zoom switch specifies which action to take
	when you use the cursor to click and drag across the plot area. In
	Zoom mode, clicking and dragging across part of the plot area
	will zoom in on the selected region. In Pan mode, clicking and
	dragging moves the plot area under the cursor to a new position.
	The view can be returned to the default view by clicking the
	Reset Graph button.
Absorbance trace	In addition to the zoom feature which scales the plot area both
	horizontally and vertically, the scale of the absorbance trace can
	be modified alone. To change the left Y-axis Scale, click and select
	a new value from the pull-down list.
Channel positions	Channel positions can be displayed in the plot area individually
	or collectively. Highlight the channels you wish to display. If mul-
	tiple absorbance traces and gradients are selected, they will be
	overlaid in the plot area. This can be useful when comparing
	channels, especially when desiring to monitor the effects of any
	experimental interventions.
Status bar	The status bar is located at the bottom edge of the BioOptix 10
	window. The fields in this bar display system information and
	messages during the run.

3.7.3Suspending System The system automatically stops after completing all blocks.

Operation	However, the experiment can be paused at any time by clicking
	the Stop button. This stops the pumps which allows you to refill
	or change buffers, or make other mechanical adjustments to the
	system. Click the Play button to resume the experiment.

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