Teledyne 10 manual Introduction, Product Overview

Page 13

BioOptix™ 10

Section 1 Introduction

1.1 Product Overview

The BioOptix™ 10 system performs automated protein purifi- cation on as many as ten liquid samples simultaneously. The system has ten independent pumps that keep flow rates properly regulated in each channel, and ten independent detectors so that you can monitor the separation in every channel. The purified sample is collected by a fraction collector — up to 300 fractions per sample for unattended operation, or unlimited fractions for attended purification runs. BioOptix 10 control software lets you quickly locate the fractions of interest.

System highlights include:

•Individual detector on each channel.

•Unique gradient conditions can be used for each channel—great for scouting ideal gradient conditions.

•Repeatable, uniform flow for each sample, regardless of buffer gradient or sample consistency. Ten independent precision pumps provide flow rates from 0.5 to 12 mL/min for each channel.

•Fast and easy sample loading—individual sample intro- duction lines for each channel.

•Compact size gives you maximum capability in minimum bench space.

•High fraction capacity—

❍collect up to 300 fractions per sample completely unattended,

❍or unlimited fractions by manually replacing filled racks during a run.

•Pumps designed for salt buffers and common solvents ensure long life, low maintenance, no seal rinse fluid needed.

1-1

Image 13

Contents BioOptix Page Foreword Page Application, take heed in the following general warnings General WarningsDamage Symboles de sécurité Hazard SymbolsBioOptix 10 Safety BioOptix Table of ContentsAppendix B Fluid Path Diagrams System MaintenanceAppendix C Replacement Parts Lists Appendix a Method TemplatesList of Figures List of Tables Product Overview IntroductionMaximum Altitude 2000 meters BioOptix 10 SpecificationsBioOptix 10 Front Panel Controls System Controls and ConnectorsBioOptix 10 Front Panel Controls BioOptix 10 Left-side Controls and Connections BioOptix 10 Left-side ConnectionsBioOptix 10 Right-side Connections BioOptix 10 Right-side ConnectionsBioOptix 10 Rear Panel Controls and Connections BioOptix 10 Rear Panel Controls and ConnectionsBioOptix 10 Installation InstallationUnpacking the BioOptix 10 System SpaceAssembling ElectricalBioOptix Accessing pump and column module internal connections Routing the Sample Out lines column module P110 Connection on BD3 AC power connection from pump module 10 Liquid connections pump module shown 11 Aligning the fasteners Foxy Ceeding with the arm assembly instructionsBioOptix Installation Common Buffer Inlet Mounting Block Manifold Channel Numbers Color Part Number Tubing Color chart16 Foxy 200 tubing connections Windows PC Minimum Requirements ConfiguringBioOptix 10 Software BioOptix 10 System Preparation 18 Column bypass tubing 20 BioOptix 10 Manual Hardware Control window BioOptix Installation Operation Sample Preparation Column InstallationAdapters Gradients BioOptix 10 SoftwareOverview Defining a Gradient To define a gradient MethodsEquilibrate tab settings Load tab settings Load Settings This block loads the sample onto the columnWash Settings Experiments Flush SettingsSetting up Experiment RunningOperation Buttons FileOperation Zoom and panPump Flush Post-experiment OperationPreview Saving Run DataAutoReports BioOptix 10 Operation Vent damage System MaintenanceExterior Cleaning Piston Seal Replacement Refer to -3 for the following steps Pump assembly components Deuterium Lamp Replacement Waste port mounting plate Common Problems TroubleshootingOperating Problems Inaccessible Fuses Circuit breaker location How to Ship Returns Service DepartmentTeledyne Isco, Inc Appendix a Method Templates Dard.tpl General Editing Rules Modifying TemplateBlockn BlocksOther Fluid-type Blocks Appendix B Fluid Path DiagramsLoad-type Blocks BioOptix Appendix B Fluid Path DiagramsFigure B-4 Fluid path for Load-type blocks Manual injections Pump Flush Plumbing DiagramFigure B-7 BioOptix 10 Plumbing Diagram BioOptix Appendix B Fluid Path Diagrams Appendix C Replacement Parts Lists BioOptix Appendix C Replacement Parts Lists Detector Assembly 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists Column Module 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists Pump Module 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists Declaration of Conformity Page Teledyne Isco One Year Limited Factory Service Warranty