Teledyne manual BioOptix 10 System Preparation

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BioOptix™ 10 Section 2 Installation

2.4BioOptix 10 System Preparation

❍Rack Change Bell – When this option is enabled, the system provides an audible signal while it is waiting for the operator to change the tube rack.

❍Com Port – select the Com Port number to which the “Y” Serial cable is connected to the PC. Refer to the documentation provided with your PC.

❍Fraction Size – type or select the desired fraction size to be collected by the Foxy 200.

❍Stroke Volume – type or select the desired stroke volume of the pump.

A larger stroke volume will reduced the number of Fill/pump cycles. However, this will limit the possible sample and pumped volumes to a multiple of the stroke volume. A stroke volume of 1 mL allows sample and pumped volumes in increments of 1 mL.

❍Refill Rate – type or select the desired Refill Rate in mL/minute.

❍Pressure Limit – type or select the desired operating pressure limit, in psi. When exceeded, the system will reduce the flow rate until the pressure returns to the normal range.

This empirical value will vary according to the proper- ties of the stationary and mobile phase. Select a value low enough to limit the flow rate when a column or tub- ing blockage may be indicated, but high enough to avoid false indications when higher column operating pressures may be the norm.

❍Default Data Directory – Click the Browse button to select a default data directory.

4.Click the Save button to accept the configuration settings and close the dialog box.

The system preparation assumes that the instructions in sec- tions 2.1 through 2.3 have been completed. System preparation primes the tubing with buffer solution to ready the BioOptix 10 for a system verification test run.

To prepare the system:

1.Ensure that BioOptix 10 software is configured and run- ning on the connected Windows PC.

2.Turn the Power switch on the left side of the BioOptix 10 to On.

3.Press the Power button on the Foxy 200.

4.Place the Buffer A inlet line(s) into two liters of solution (such as HPLC-grade water or other appropriate buffer solution).

5.Place the Buffer B inlet line(s) into two liters of solution (such as HPLC-grade water or other appropriate buffer solution).

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Contents BioOptix Page Foreword Page Application, take heed in the following general warnings General WarningsDamage Symboles de sécurité Hazard SymbolsBioOptix 10 Safety BioOptix Table of ContentsAppendix B Fluid Path Diagrams System MaintenanceAppendix C Replacement Parts Lists Appendix a Method TemplatesList of Figures List of Tables Product Overview IntroductionMaximum Altitude 2000 meters BioOptix 10 SpecificationsSystem Controls and Connectors BioOptix 10 Front Panel ControlsBioOptix 10 Front Panel Controls BioOptix 10 Left-side Controls and Connections BioOptix 10 Left-side ConnectionsBioOptix 10 Right-side Connections BioOptix 10 Right-side ConnectionsBioOptix 10 Rear Panel Controls and Connections BioOptix 10 Rear Panel Controls and ConnectionsBioOptix 10 Installation InstallationUnpacking the BioOptix 10 System SpaceElectrical AssemblingBioOptix Accessing pump and column module internal connections Routing the Sample Out lines column module P110 Connection on BD3 AC power connection from pump module 10 Liquid connections pump module shown 11 Aligning the fasteners Foxy Ceeding with the arm assembly instructionsBioOptix Installation Common Buffer Inlet Mounting Block Manifold Channel Numbers Color Part Number Tubing Color chart16 Foxy 200 tubing connections Configuring Windows PC Minimum RequirementsBioOptix 10 Software BioOptix 10 System Preparation 18 Column bypass tubing 20 BioOptix 10 Manual Hardware Control window BioOptix Installation Sample Preparation Column Installation OperationAdapters BioOptix 10 Software GradientsOverview Defining a Gradient To define a gradient MethodsEquilibrate tab settings Load tab settings Load Settings This block loads the sample onto the columnWash Settings Experiments Flush SettingsSetting up Experiment RunningOperation Buttons FileOperation Zoom and panPump Flush Post-experiment OperationPreview Saving Run DataAutoReports BioOptix 10 Operation Vent damage System MaintenanceExterior Cleaning Piston Seal Replacement Refer to -3 for the following steps Pump assembly components Deuterium Lamp Replacement Waste port mounting plate Troubleshooting Common ProblemsOperating Problems Inaccessible Fuses Circuit breaker location Service Department How to Ship ReturnsTeledyne Isco, Inc Appendix a Method Templates Dard.tpl General Editing Rules Modifying TemplateBlocks BlocknOther Fluid-type Blocks Appendix B Fluid Path DiagramsLoad-type Blocks BioOptix Appendix B Fluid Path DiagramsFigure B-4 Fluid path for Load-type blocks Manual injections Pump Flush Plumbing DiagramFigure B-7 BioOptix 10 Plumbing Diagram BioOptix Appendix B Fluid Path Diagrams Appendix C Replacement Parts Lists BioOptix Appendix C Replacement Parts Lists Detector Assembly 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists Column Module 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists Pump Module 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists Declaration of Conformity Page Teledyne Isco One Year Limited Factory Service Warranty