Teledyne 10 manual Equilibrate tab settings

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BioOptix™ 10

Section 3 Operation

The default method file contains five blocks that are appropriate for use with affinity column purification:

Equilibrate – conditions the column.

Load – loads the sample onto the column.

Wash – washes compounds from the column that do not bind to the column media.

Elute – pumps a buffer solution to release the purified compounds from the column.

Post Run– flushes any remaining compounds from the column to prepare the column for the next purification run, storage, or disposal.

3.5.1Equilibrate Settings This block conditions the column and fills the column with the desired buffer solution for starting the purification.

Figure 3-2 Equilibrate tab settings

The Equilibrate tab (Figure 3-2) contains the following:

Fluid, %B – Enter the desired mix of buffer fluid as a percentage of Buffer B.

Flow Rate, mL/min – Enter the desired flow rate, from 0.5 to 12 mL/min.

Volume, mL – Enter the total amount of Buffer fluid to pump during this block.

Collect Effluent – Select the desired action for effluent fluid as it comes off the column. Selecting No diverts the effluent to a channel waste port. Selecting Yes sends the fluid to the fraction collector. If Yes is selected, enter a fraction size in mL. The fraction collector will advance to the next row of tubes after collecting the entered volume.

Pause at End – Select Yes to have the system wait for operator intervention after completing this block. Select No to automatically advance to the next block.

3-4

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Contents BioOptix Page Foreword Page General Warnings Application, take heed in the following general warningsDamage Hazard Symbols Symboles de sécuritéBioOptix 10 Safety Table of Contents BioOptixSystem Maintenance Appendix B Fluid Path DiagramsAppendix C Replacement Parts Lists Appendix a Method TemplatesList of Figures List of Tables Introduction Product OverviewBioOptix 10 Specifications Maximum Altitude 2000 metersBioOptix 10 Front Panel Controls System Controls and ConnectorsBioOptix 10 Front Panel Controls BioOptix 10 Left-side Connections BioOptix 10 Left-side Controls and ConnectionsBioOptix 10 Right-side Connections BioOptix 10 Right-side ConnectionsBioOptix 10 Rear Panel Controls and Connections BioOptix 10 Rear Panel Controls and ConnectionsInstallation BioOptix 10 InstallationUnpacking the BioOptix 10 System SpaceAssembling ElectricalBioOptix Accessing pump and column module internal connections Routing the Sample Out lines column module P110 Connection on BD3 AC power connection from pump module 10 Liquid connections pump module shown 11 Aligning the fasteners Ceeding with the arm assembly instructions FoxyBioOptix Installation Common Buffer Inlet Mounting Block Manifold Tubing Color chart Channel Numbers Color Part Number16 Foxy 200 tubing connections Windows PC Minimum Requirements ConfiguringBioOptix 10 Software BioOptix 10 System Preparation 18 Column bypass tubing 20 BioOptix 10 Manual Hardware Control window BioOptix Installation Operation Sample Preparation Column InstallationAdapters Gradients BioOptix 10 SoftwareOverview Methods Defining a Gradient To define a gradientEquilibrate tab settings Load Settings This block loads the sample onto the column Load tab settingsWash Settings Flush Settings ExperimentsSetting up Running ExperimentOperation Buttons FileZoom and pan OperationPost-experiment Operation Pump FlushSaving Run Data PreviewAutoReports BioOptix 10 Operation System Maintenance Vent damageExterior Cleaning Piston Seal Replacement Refer to -3 for the following steps Pump assembly components Deuterium Lamp Replacement Waste port mounting plate Common Problems TroubleshootingOperating Problems Inaccessible Fuses Circuit breaker location How to Ship Returns Service DepartmentTeledyne Isco, Inc Appendix a Method Templates Dard.tpl Modifying Template General Editing RulesBlockn BlocksOther Appendix B Fluid Path Diagrams Fluid-type BlocksBioOptix Appendix B Fluid Path Diagrams Load-type BlocksFigure B-4 Fluid path for Load-type blocks Manual injections Plumbing Diagram Pump FlushFigure B-7 BioOptix 10 Plumbing Diagram BioOptix Appendix B Fluid Path Diagrams Appendix C Replacement Parts Lists BioOptix Appendix C Replacement Parts Lists Detector Assembly 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists Column Module 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists Pump Module 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists Declaration of Conformity Page Teledyne Isco One Year Limited Factory Service Warranty