BioOptix™ 10
Section 3 Operation
The default method file contains five blocks that are appropriate for use with affinity column purification:
•Equilibrate – conditions the column.
•Load – loads the sample onto the column.
•Wash – washes compounds from the column that do not bind to the column media.
•Elute – pumps a buffer solution to release the purified compounds from the column.
•Post Run– flushes any remaining compounds from the column to prepare the column for the next purification run, storage, or disposal.
3.5.1Equilibrate Settings This block conditions the column and fills the column with the desired buffer solution for starting the purification.
Figure 3-2 Equilibrate tab settings
The Equilibrate tab (Figure
•Fluid, %B – Enter the desired mix of buffer fluid as a percentage of Buffer B.
•Flow Rate, mL/min – Enter the desired flow rate, from 0.5 to 12 mL/min.
•Volume, mL – Enter the total amount of Buffer fluid to pump during this block.
•Collect Effluent – Select the desired action for effluent fluid as it comes off the column. Selecting No diverts the effluent to a channel waste port. Selecting Yes sends the fluid to the fraction collector. If Yes is selected, enter a fraction size in mL. The fraction collector will advance to the next row of tubes after collecting the entered volume.
•Pause at End – Select Yes to have the system wait for operator intervention after completing this block. Select No to automatically advance to the next block.