Teledyne 10 manual Running, Experiment, Operation Buttons, File, System Operation

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BioOptix™ 10

Section 3 Operation

3.6.3Saving the Experiment Save the experiment by selecting the File>Save menu command.

File

Use a descriptive file name so that it can be located for later use.

3.7 Running the

With the experiment file open, some BioOptix 10 operation

Experiment

buttons along the bottom of the window are active (Figure 3-8).

 

Buttons will be active or inactive according to the current state of

 

the system.

 

 

 

 

 

Fast

Next

 

 

Play

Stop Forward

Tube

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Figure 3-8 BioOptix 10 Operation Buttons

 

Table 3-1 Operation Buttons

 

 

 

 

Button

Description

 

Play

Click to begin the experiment run. If the Stop

 

 

button was clicked during the run, clicking the

 

 

Play button will resume the run.

 

 

 

 

Stop

Click to suspend the run activity. From this state

 

 

you click Play to resume the run, or click Fast

 

 

Forward to skip to the next block or abort the

 

 

run.

 

 

 

 

Fast Forward

Click the Fast Forward to skip to the next block

 

 

or abort the run.

 

 

 

 

Next Tube

Click this button to advance the fraction collector

 

 

to the next row. This is useful when there is a

 

 

need to isolate effluent.

 

 

 

 

AutoZero

The baseline absorbance trace may shift if

 

 

buffer solutions are changed mid-run. Click this

 

 

button to re-zero the absorbance trace.

 

 

 

 

Show Run Window

Click this button to bring the experiment window

 

 

for the run in progress to the foreground. If you

 

 

have several other files (e.g. previous experi-

 

 

ments or new methods) open in the BioOptix 10

 

 

window this button lets you quickly return to the

 

 

running experiment.

 

 

 

3.7.1 System Operation

To start the experiment, click the Play button. The system begins

 

operation with the first block and proceeds according to the

 

experiment and method files.

Referring to Figure 3-7, this first block would be “Equilibrate” and use the operating parameters specified by the method file (Figure 3-2). The system would pump 0 %B (100% of buffer A) at

5.0mL/min. The system would pump 50 mL and then pause (Figure 3-9). Similarly, the BioOptix 10 would proceed through the Sample Load, Wash, Elute, and Flush blocks.

3-9

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Contents BioOptix Page Foreword Page Application, take heed in the following general warnings General WarningsDamage Symboles de sécurité Hazard SymbolsBioOptix 10 Safety BioOptix Table of ContentsAppendix B Fluid Path Diagrams System MaintenanceAppendix C Replacement Parts Lists Appendix a Method TemplatesList of Figures List of Tables Product Overview IntroductionMaximum Altitude 2000 meters BioOptix 10 SpecificationsSystem Controls and Connectors BioOptix 10 Front Panel ControlsBioOptix 10 Front Panel Controls BioOptix 10 Left-side Controls and Connections BioOptix 10 Left-side ConnectionsBioOptix 10 Right-side Connections BioOptix 10 Right-side ConnectionsBioOptix 10 Rear Panel Controls and Connections BioOptix 10 Rear Panel Controls and ConnectionsBioOptix 10 Installation InstallationUnpacking the BioOptix 10 System SpaceElectrical AssemblingBioOptix Accessing pump and column module internal connections Routing the Sample Out lines column module P110 Connection on BD3 AC power connection from pump module 10 Liquid connections pump module shown 11 Aligning the fasteners Foxy Ceeding with the arm assembly instructionsBioOptix Installation Common Buffer Inlet Mounting Block Manifold Channel Numbers Color Part Number Tubing Color chart16 Foxy 200 tubing connections Configuring Windows PC Minimum RequirementsBioOptix 10 Software BioOptix 10 System Preparation 18 Column bypass tubing 20 BioOptix 10 Manual Hardware Control window BioOptix Installation Sample Preparation Column Installation OperationAdapters BioOptix 10 Software GradientsOverview Defining a Gradient To define a gradient MethodsEquilibrate tab settings Load tab settings Load Settings This block loads the sample onto the columnWash Settings Experiments Flush SettingsSetting up Experiment RunningOperation Buttons FileOperation Zoom and panPump Flush Post-experiment OperationPreview Saving Run DataAutoReports BioOptix 10 Operation Vent damage System MaintenanceExterior Cleaning Piston Seal Replacement Refer to -3 for the following steps Pump assembly components Deuterium Lamp Replacement Waste port mounting plate Troubleshooting Common ProblemsOperating Problems Inaccessible Fuses Circuit breaker location Service Department How to Ship ReturnsTeledyne Isco, Inc Appendix a Method Templates Dard.tpl General Editing Rules Modifying TemplateBlocks BlocknOther Fluid-type Blocks Appendix B Fluid Path DiagramsLoad-type Blocks BioOptix Appendix B Fluid Path DiagramsFigure B-4 Fluid path for Load-type blocks Manual injections Pump Flush Plumbing DiagramFigure B-7 BioOptix 10 Plumbing Diagram BioOptix Appendix B Fluid Path Diagrams Appendix C Replacement Parts Lists BioOptix Appendix C Replacement Parts Lists Detector Assembly 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists Column Module 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists Pump Module 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists Declaration of Conformity Page Teledyne Isco One Year Limited Factory Service Warranty