Teledyne 10 manual Running, Experiment, Operation Buttons, File, System Operation

BioOptix™ 10

Section 3 Operation

3.6.3Saving the Experiment Save the experiment by selecting the File>Save menu command.

File	Use a descriptive file name so that it can be located for later use.
3.7 Running the	With the experiment file open, some BioOptix 10 operation
Experiment	buttons along the bottom of the window are active (Figure 3-8).
	Buttons will be active or inactive according to the current state of
	the system.
			Fast	Next
		Play	Stop Forward	Tube

Figure 3-8 BioOptix 10 Operation Buttons

	Table 3-1 Operation Buttons
	Button	Description
	Play	Click to begin the experiment run. If the Stop
		button was clicked during the run, clicking the
		Play button will resume the run.
	Stop	Click to suspend the run activity. From this state
		you click Play to resume the run, or click Fast
		Forward to skip to the next block or abort the
		run.
	Fast Forward	Click the Fast Forward to skip to the next block
		or abort the run.
	Next Tube	Click this button to advance the fraction collector
		to the next row. This is useful when there is a
		need to isolate effluent.
	AutoZero	The baseline absorbance trace may shift if
		buffer solutions are changed mid-run. Click this
		button to re-zero the absorbance trace.
	Show Run Window	Click this button to bring the experiment window
		for the run in progress to the foreground. If you
		have several other files (e.g. previous experi-
		ments or new methods) open in the BioOptix 10
		window this button lets you quickly return to the
		running experiment.
3.7.1 System Operation	To start the experiment, click the Play button. The system begins
	operation with the first block and proceeds according to the
	experiment and method files.

Referring to Figure 3-7, this first block would be “Equilibrate” and use the operating parameters specified by the method file (Figure 3-2). The system would pump 0 %B (100% of buffer A) at

5.0mL/min. The system would pump 50 mL and then pause (Figure 3-9). Similarly, the BioOptix 10 would proceed through the Sample Load, Wash, Elute, and Flush blocks.

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