BioOptix™ 10
Section 3 Operation
Pausing at the end of a block allows for buffer changes or other mechanical adjustments before proceeding with the next block.
•Wavelength – Type or select the desired wavelength from 190 to 360 nm to be used for UV detection. Note that all blocks use the same wavelength.
Note
The Equilibrate tab is a “Fluid” block type. See Appendix A.
3.5.2 Load Settings | This block loads the sample onto the column. | |
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Figure 3-3 Load tab settings
The Sample Load tab (Figure
•Injection – Select Pump to pump liquid sample from the Sample In ports onto the column. Select Manual to inject the sample from the manual injection ports (located on the top of the BioOptix 10) onto the column. Note that selecting Manual disables the flow rate and volume settings.
•Flow Rate, mL/min – If a Pump injection is selected, enter the desired flow rate in mL/min.
•Volume – If a Pump injection is selected, enter the desired volume in mL.
•Collect Effluent – Select the desired action for effluent fluid as it comes off the column. If Yes is selected, enter the desired fraction size.
•Pause at End – Select Yes to have the system wait for operator intervention after completing this block. Select No to automatically advance to the next block.
Note
The Load tab is a “Load” block type. See Appendix A.