Teledyne 10 manual Troubleshooting, Common Problems, Operating Problems

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BioOptix™ 10

Section 4 System Maintenance

Replacing the lamp

To install the new lamp:

CAUTION

4.5 Troubleshooting

Contaminants on the glass bulb will shorten the life span. Oil from your skin is one such contaminant. Wear clean cotton gloves when handling the deuterium lamp.

1.Carefully insert the replacement bulb (part number

69-5203-014) into the optical assembly and secure it with the lamp mounting hardware.

2.Route and secure the wires through the twist lock clamp.

3.Reconnect the wiring connector.

4.Replace the waste port mounting plate and secure it with the hardware.

Overall performance troubleshooting can be simplified if you maintain a system log that includes the number and type of samples that are separated.

If any difficulties or abnormal results are noted, they should be indicated in the log to assist in diagnosing any system problems.

It is worthwhile to maintain a standard sample that can be run periodically to check the overall performance of the system.

If you notice in your log that detector responses have changed, or you are running samples and the absorbance readings are not what you think they should be, you should run a standard.

If you run a standard sample and obtain the expected results, the problem is not being caused by the instrument. You can then examine other causes for your problem, such as the column or mobile phase.

4.5.1 Operating Problems

Table 4-1 Common Problems

Problem	Causes
Overpressure on one or more channels	Sample precipitated (crashed) in column or solid phase cartridge.
	Tubing is kinked.
	Injection valve is set at an incorrect position.

No peaks detected	Incorrect wavelength.

Dripping in unused column positions	Lines not purged after last use.
	Slight leakage from contaminants in check valves. This is usually not detri-
	mental to performance. Column bypass tubes should be used on unused
	channels.

System will not prime	Prime all channels to overcome the added minute leakage in each channel.
	Make sure the bypass tubes are in place on all channels.
	Inlet lines clogged; insufficient filtering.
	Inlet lines are not securely connected.
	Air leakage at internal fitting.

4-7

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Contents BioOptix Page Foreword Page Application, take heed in the following general warnings General WarningsDamage Symboles de sécurité Hazard SymbolsBioOptix 10 Safety BioOptix Table of ContentsAppendix B Fluid Path Diagrams System MaintenanceAppendix C Replacement Parts Lists Appendix a Method TemplatesList of Figures List of Tables Product Overview IntroductionMaximum Altitude 2000 meters BioOptix 10 SpecificationsSystem Controls and Connectors BioOptix 10 Front Panel ControlsBioOptix 10 Front Panel Controls BioOptix 10 Left-side Controls and Connections BioOptix 10 Left-side ConnectionsBioOptix 10 Right-side Connections BioOptix 10 Right-side ConnectionsBioOptix 10 Rear Panel Controls and Connections BioOptix 10 Rear Panel Controls and ConnectionsBioOptix 10 Installation InstallationUnpacking the BioOptix 10 System SpaceElectrical AssemblingBioOptix Accessing pump and column module internal connections Routing the Sample Out lines column module P110 Connection on BD3 AC power connection from pump module 10 Liquid connections pump module shown 11 Aligning the fasteners Foxy Ceeding with the arm assembly instructionsBioOptix Installation Common Buffer Inlet Mounting Block Manifold Channel Numbers Color Part Number Tubing Color chart16 Foxy 200 tubing connections Configuring Windows PC Minimum RequirementsBioOptix 10 Software BioOptix 10 System Preparation 18 Column bypass tubing 20 BioOptix 10 Manual Hardware Control window BioOptix Installation Sample Preparation Column Installation OperationAdapters BioOptix 10 Software GradientsOverview Defining a Gradient To define a gradient MethodsEquilibrate tab settings Load tab settings Load Settings This block loads the sample onto the columnWash Settings Experiments Flush SettingsSetting up Experiment RunningOperation Buttons FileOperation Zoom and panPump Flush Post-experiment OperationPreview Saving Run DataAutoReports BioOptix 10 Operation Vent damage System MaintenanceExterior Cleaning Piston Seal Replacement Refer to -3 for the following steps Pump assembly components Deuterium Lamp Replacement Waste port mounting plate Troubleshooting Common ProblemsOperating Problems Inaccessible Fuses Circuit breaker location Service Department How to Ship ReturnsTeledyne Isco, Inc Appendix a Method Templates Dard.tpl General Editing Rules Modifying TemplateBlocks BlocknOther Fluid-type Blocks Appendix B Fluid Path DiagramsLoad-type Blocks BioOptix Appendix B Fluid Path DiagramsFigure B-4 Fluid path for Load-type blocks Manual injections Pump Flush Plumbing DiagramFigure B-7 BioOptix 10 Plumbing Diagram BioOptix Appendix B Fluid Path Diagrams Appendix C Replacement Parts Lists BioOptix Appendix C Replacement Parts Lists Detector Assembly 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists Column Module 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists Pump Module 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists Declaration of Conformity Page Teledyne Isco One Year Limited Factory Service Warranty