Teledyne 10 manual Experiments, Flush Settings

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BioOptix™ 10

Section 3 Operation

•Collect Effluent – Select the desired action for effluent fluid as it comes off the column. If Yes is selected, enter the desired fraction size.

		Note
		The Elute tab is a “Gradient” block type. See Appendix A.
3.5.5 Flush Settings	This block pumps fluid to flush remaining sample from the
	column and system tubing. This step can prepare the column for
	the next purification run, storage, or disposal.
	The Flush tab is a fluid block type, therefore the settings dis-
	played on this tab are the same as the Equilibrate and Wash
	tabs. Refer to section 3.5.1 for a discussion on the settings for this
	tab.

Figure 3-6 Flush tab settings

3.5.6Saving the Method File To be used in an experiment file, the method file must be saved. Save the method by selecting the File>Save menu command. Use a descriptive file name so that it can be located later.

3.6 Experiments

An experiment file can be created by:

•selecting the File>New>Experiment menu command,

•pressing the Ctrl+E keyboard buttons,

•or by clicking the E toolbar button.

Before building and displaying the experiment file, you must select a method file that will be used for all ten channels. In the Select a Method for Experiment dialog box, locate and highlight the method file, then click the Open button.

An experiment file displays operating parameters in a table. Many of the columns in the table are determined by the template file used to create the method file. (Refer to Appendix A for more details on the template file.) Default values will be assigned to the table cells from the method file you’ve chosen.

3-7

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Contents BioOptix Page Foreword Page Application, take heed in the following general warnings General WarningsDamage Symboles de sécurité Hazard SymbolsBioOptix 10 Safety BioOptix Table of ContentsAppendix a Method Templates System MaintenanceAppendix B Fluid Path Diagrams Appendix C Replacement Parts ListsList of Figures List of Tables Product Overview IntroductionMaximum Altitude 2000 meters BioOptix 10 SpecificationsBioOptix 10 Front Panel Controls System Controls and ConnectorsBioOptix 10 Front Panel Controls BioOptix 10 Left-side Controls and Connections BioOptix 10 Left-side ConnectionsBioOptix 10 Right-side Connections BioOptix 10 Right-side ConnectionsBioOptix 10 Rear Panel Controls and Connections BioOptix 10 Rear Panel Controls and ConnectionsSpace InstallationBioOptix 10 Installation Unpacking the BioOptix 10 SystemAssembling ElectricalBioOptix Accessing pump and column module internal connections Routing the Sample Out lines column module P110 Connection on BD3 AC power connection from pump module 10 Liquid connections pump module shown 11 Aligning the fasteners Foxy Ceeding with the arm assembly instructionsBioOptix Installation Common Buffer Inlet Mounting Block Manifold Channel Numbers Color Part Number Tubing Color chart16 Foxy 200 tubing connections Windows PC Minimum Requirements ConfiguringBioOptix 10 Software BioOptix 10 System Preparation 18 Column bypass tubing 20 BioOptix 10 Manual Hardware Control window BioOptix Installation Operation Sample Preparation Column InstallationAdapters Gradients BioOptix 10 SoftwareOverview Defining a Gradient To define a gradient MethodsEquilibrate tab settings Load tab settings Load Settings This block loads the sample onto the columnWash Settings Experiments Flush SettingsSetting up File RunningExperiment Operation ButtonsOperation Zoom and panPump Flush Post-experiment OperationPreview Saving Run DataAutoReports BioOptix 10 Operation Vent damage System MaintenanceExterior Cleaning Piston Seal Replacement Refer to -3 for the following steps Pump assembly components Deuterium Lamp Replacement Waste port mounting plate Common Problems TroubleshootingOperating Problems Inaccessible Fuses Circuit breaker location How to Ship Returns Service DepartmentTeledyne Isco, Inc Appendix a Method Templates Dard.tpl General Editing Rules Modifying TemplateBlockn BlocksOther Fluid-type Blocks Appendix B Fluid Path DiagramsLoad-type Blocks BioOptix Appendix B Fluid Path DiagramsFigure B-4 Fluid path for Load-type blocks Manual injections Pump Flush Plumbing DiagramFigure B-7 BioOptix 10 Plumbing Diagram BioOptix Appendix B Fluid Path Diagrams Appendix C Replacement Parts Lists BioOptix Appendix C Replacement Parts Lists Detector Assembly 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists Column Module 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists Pump Module 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists Declaration of Conformity Page Teledyne Isco One Year Limited Factory Service Warranty