Teledyne manual BioOptix 10 Software, Gradients, Overview

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BioOptix™ 10

Section 3 Operation

3.3 BioOptix 10 Software

			Note
			Refer to On-line Help for specific BioOptix 10 software operat-
			ing instructions.
3.3.1 Overview		The operation of the BioOptix 10 system is directed by three
		files:
			• Gradient – these files define how a gradient is to be
			formed from Buffer A and B solutions.
			• Method – these files contain the settings that direct the
			operation of each channel of the system. A method file
			will reference the settings in a gradient file.
			• Experiment – These files contain settings that direct the
			operation of all channels. Experiment files are based on
			method file settings. The BioOptix 10 system operation
			can be started after the experiment settings are defined.
		Sections 3.4 through 3.6 describe these files in detail.
3.4 Gradients		A gradient file can be created by:
			• selecting the File>New>Gradient menu command,
			• pressing the Ctrl+G keyboard buttons,
			• or by clicking the G toolbar button.
		A new gradient file displays an empty two-column table and a
		plot area (Figure 3-1). The table contains inflection points —
			points at which the rate of gradient formation change.
		The Initial volume row in the table defines the starting mixture,
		expressed as percent B.

Figure 3-1 Gradient File

3-2

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Contents BioOptix Page Foreword Page General Warnings Application, take heed in the following general warningsDamage Hazard Symbols Symboles de sécuritéBioOptix 10 Safety Table of Contents BioOptixAppendix C Replacement Parts Lists System MaintenanceAppendix B Fluid Path Diagrams Appendix a Method TemplatesList of Figures List of Tables Introduction Product OverviewBioOptix 10 Specifications Maximum Altitude 2000 metersBioOptix 10 Front Panel Controls System Controls and ConnectorsBioOptix 10 Front Panel Controls BioOptix 10 Left-side Connections BioOptix 10 Left-side Controls and ConnectionsBioOptix 10 Right-side Connections BioOptix 10 Right-side ConnectionsBioOptix 10 Rear Panel Controls and Connections BioOptix 10 Rear Panel Controls and ConnectionsUnpacking the BioOptix 10 System InstallationBioOptix 10 Installation SpaceBioOptix ElectricalAssembling Accessing pump and column module internal connections Routing the Sample Out lines column module P110 Connection on BD3 AC power connection from pump module 10 Liquid connections pump module shown 11 Aligning the fasteners Ceeding with the arm assembly instructions FoxyBioOptix Installation Common Buffer Inlet Mounting Block Manifold Tubing Color chart Channel Numbers Color Part Number16 Foxy 200 tubing connections BioOptix 10 Software ConfiguringWindows PC Minimum Requirements BioOptix 10 System Preparation 18 Column bypass tubing 20 BioOptix 10 Manual Hardware Control window BioOptix Installation Adapters Sample Preparation Column InstallationOperation Overview BioOptix 10 SoftwareGradients Methods Defining a Gradient To define a gradientEquilibrate tab settings Load Settings This block loads the sample onto the column Load tab settingsWash Settings Flush Settings ExperimentsSetting up Operation Buttons RunningExperiment FileZoom and pan OperationPost-experiment Operation Pump FlushSaving Run Data PreviewAutoReports BioOptix 10 Operation System Maintenance Vent damageExterior Cleaning Piston Seal Replacement Refer to -3 for the following steps Pump assembly components Deuterium Lamp Replacement Waste port mounting plate Operating Problems TroubleshootingCommon Problems Inaccessible Fuses Circuit breaker location Teledyne Isco, Inc Service DepartmentHow to Ship Returns Appendix a Method Templates Dard.tpl Modifying Template General Editing RulesOther BlocksBlockn Appendix B Fluid Path Diagrams Fluid-type BlocksBioOptix Appendix B Fluid Path Diagrams Load-type BlocksFigure B-4 Fluid path for Load-type blocks Manual injections Plumbing Diagram Pump FlushFigure B-7 BioOptix 10 Plumbing Diagram BioOptix Appendix B Fluid Path Diagrams Appendix C Replacement Parts Lists BioOptix Appendix C Replacement Parts Lists Detector Assembly 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists Column Module 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists Pump Module 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists 13BioOptix 10 Replacement Parts Lists Declaration of Conformity Page Teledyne Isco One Year Limited Factory Service Warranty