Leica CM3050 S instruction manual Infectious/radioactive material, Disinfection and cleaning

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2.Safety

2.3.9 Infectious/radioactive material

•Danger!

Use caution when working with potentially infectious specimens:

-->Risk of infection!

•When working with potentially infectious / radioactive specimens:

•Wear protective clothes (gloves, protec- tive boots, mask, lab coat), in compliance with radiation safety regulations and/or in-house regulations on handling infec- tious / radioactive material.

•When working with radioactive specimens:

•Comply with applicable radiation safety regulations!

•Dispose of radioactive specimen waste ac- cording to applicable regulations.

2.3.10 Disinfection and cleaning

•Prior to disinfection, switch the instrument off and unplug it from mains.

•For removal of the microtome from the cryo- chamber see chapter 2.3.11 ‘ Removing the microtome’.

•For disinfection, wear protective gear: (gloves, mask, lab coat etc.)!

•For disinfection, only use alcohol-based dis- infectants!

•Do not use solvents (xylene, acetone etc.) for cleaning or disinfection!

•Do not spray disinfectants into the evapora- tor!

-->Risk of icing!

•Explosion hazard when working with alcohol: Make sure the premises are appropriately ventilated!

•When using disinfectants and detergents, comply with all safety instructions supplied by the manufacturer of the product!

•Dispose of waste liquids from disinfection/ cleaning as well as of sectioning waste ac- cording to applicable regulations on disposal of special category waste!

•Disinfected accessories must be thoroughly dry when reinserting them into the chamber!

-->Risk of icing!

•Make sure the chamber is completely dry before switching the instrument back on:

-->Explosion hazard through alcohol vapors! -->Risk of icing!

14	Instruction Manual V1.2 - 11/2006

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Contents Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Serial- number Symbols used in this manual and their meaningImportant information Qualification of personnel GeneralDesignated use Any other use of the instrument is considered improperIntegrated safety devices SicherheitLocking SafetyLocking the handwheel UnlockingKnife guard Activating the emergency stop functionDeactivating the emergency stop Site requirements Electrical connectionsHandling microtome knives/blades Defrosting/Handling frozen tissueFrozen parts of the instrument and frozen accessories Knife guard/handwheel lockDisinfection and cleaning Infectious/radioactive materialDisplay message ‘Dry microtome’ 11Removing/reinstalling the microtomeMaintenance General site requirements InstallationUnpacking and installation RepackingStandard delivery Further accessories Inserting the accessories Connecting the footswitch Footswitch FunctionModels with footswitch Prior to switching on the instrumentInstrument Properties OverviewInstrument Properties Technical Data Microtome Rotary microtome Operation Operating the instrument for the first timeMains switch / automatic cutout for the instrument Switching on/fusesSwitching on Automatic cutout for sectioning motorLock / unlock key Control panel Key functions in control panelDisplay functions in control panel Activates / deactivates a number of functionsY M I C R O T O M E Specimen temperature Instruments with specimen cooling only 15-minute stepsPress key until menu item ‘Preset counter’ is displayed Cryochamber temperaturePreset counter Instruments with sectioning motor only = Preset counter activatedDuration of defrost cycle Real timeStarting time of automatic defrost cycle Press lock/unlock key to activate or de- activate ON/OFFStatus display StopSection thickness Total section thickness counter These parameters can be displayed via the ar- row keysRefrigeration state Control panel Instruments without sectioning motor Instruments with sectioning motorStart / stop motorized sectioning Activating a sectioning modeSectioning mode 1 Intermittent stroke Press sectioning window key once more Emergency stop To start, press and hold Functional area B all models Coarse feed keysCoarse feed ‘fast forward’ Travelling speed 1,000 μm/s Motorized trimming Setting the section thicknessSelecting trimming thickness ‘Knife guard / Handwheel lock’ Chapter ‘Defrosting Handling frozen tissue’ ChapterDaily operation of the instrument Mobile heat extractor Installing the 90 prismFreezing specimens with the 90 prism See .3.4 ‘Menu functions’ itemSpecimen orientation ThermoblockInserting specimen discs into the specimen head Sectioning Trimming / sectioning Inserting the knifeManual trimming Insert the knife / disposable blade into the knife holderLock handwheel Switching off the instrument for a longer period of timeFinishing work 5.6.1 Daily routine steps Instruments with specimen cooling DefrostingAutomatic chamber defrost cycle Manual chamber defrost cycle Troubleshooting, applications tips Potential problems causes and remedies Problem Causes RemediesProblem Causes Luke-warm specimen disc Problem Causes Remedies Microtome defectiveReference chart for temperature settings in minus C TissueSpray disinfection with Leica Cryofect Disinfection, cleaning and maintenanceSafety instructions On disinfection and cleaning Allow to soak for 15 minutesDisinfection with conventional disinfectants CleaningSwitch the instrument off Remove section waste from the chamber and dispose of it‘Disinfection and cleaning’ Chapter Unlock handwheel Reinstalling the microtomePlace plastic coupling 11 on handwheel coupling Removal Installing the replacement lampExchanging the fluorescent light lamp Removing the sliding windowTechnical service information Warranty and serviceWarranty Decommissioning and disposalAppendix Electrohydraulic height adjustmentSafety instructions Installation Control keys Maintenance and careTroubleshooting Specimen discs Ordering information accessories Knife holdersAccessories ConsumablesEC Declaration of Conformity Dag Graupner Managing DirectorDear Customer This equipment has been cleaned and decontaminatedDecontamination Certificate master NB Microtome knives must be in boxesBU Return Authorisation Number