Leica Comprehensive Operation Manual for CM3050 S and Related Cryostat Models

Page 33

5.Operation

5.3.6Indication of refrigeration state, section thickness setting and counters

These parameters can be displayed via the ar- row keys.

When pressing , the parameters are dis- played in the sequence listed on this page; when pressing , they are displayed in re- verse order. The display can be scrolled contin- uously, i.e. after the last parameter the display will automatically start again with the first.

1. Refrigeration state

C

T

-

3

0

°

C

 

 

O

T

-

3

5

°

C

￿

￿

H

O

M

E

 

 

 

 

 

 

 

 

￿￿

Meaning of the refrigeration symbols (asterisks): --> see chapter 5.3.5 ‘Status indication’.

2. Section thickness

C

T

-

3

0

°

C

 

 

O

T

-

3

5

°

C

 

 

 

 

 

 

 

 

 

 

μ

0

1

0

.

0

4. Total section thickness counter

 

C

T

-

3

0

°

C

 

 

O

T

-

3

5

°

C

 

 

 

 

 

 

 

 

 

μ

0

0

0

0

.

0

Can also be set to ‘0’ at any time (via

key).

If the total section thickness indication is not set to ‘0’ manually, the instrument counts up to maximum 9999.5 μm at which point the counter will automati- cally switch back to ‘0’.

This indication shows the total thickness of all sections carried out since the counter was last set to ‘0’. (All sections, in sectioning and trim- ming mode, are counted).

5. Preset counter

C

T

-

3

0

°

C

 

 

O

T

-

3

5

°

C

 

 

 

 

 

 

 

 

 

 

 

d

n

0

5

6

Setting preset counter to ‘0’:

The sectioning thickness is set with the or key (see chapter 5.4.7 ‘Setting the section thickness’).

3. Section counter

C

T

-

3

0

°

C

 

 

O

T

-

3

5

°

C

 

 

 

 

 

 

 

 

 

 

 

n

0

0

0

Once displayed (via arrow keys), the section counter can be set to ‘0’ at any time.

• To set to ‘0’ press .

If the section counter is not set to ‘0’ by the user at some point, sections are counted up to ‘999’ at which point the counter automatically switches back to ‘0’.

Press key.

can also be pressed while the continuous sectioning mode is in motion; in this case the preset counter is set to ‘0’ and sectioning stops upon reaching the lower limit position.

After it has been set to ‘0’, the preset counter is inactive.

Reloading the former reference value:

Press and simultaneously.

Reloading the former reference value is possible also while the continuous sectioning mode is in motion:

Upon pressing both arrow keys, the instrument continues sectioning without interruption, start- ing to count down from the old reference value.

Leica CM 3050 S – Cryostat

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Contents Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Symbols used in this manual and their meaning Important informationSerial- number Designated use GeneralQualification of personnel Any other use of the instrument is considered improperSicherheit Integrated safety devicesLocking the handwheel SafetyLocking UnlockingActivating the emergency stop function Deactivating the emergency stopKnife guard Electrical connections Site requirementsFrozen parts of the instrument and frozen accessories Defrosting/Handling frozen tissueHandling microtome knives/blades Knife guard/handwheel lockInfectious/radioactive material Disinfection and cleaning11Removing/reinstalling the microtome MaintenanceDisplay message ‘Dry microtome’ Unpacking and installation InstallationGeneral site requirements RepackingStandard delivery Further accessories Inserting the accessories Models with footswitch Footswitch FunctionConnecting the footswitch Prior to switching on the instrumentOverview Instrument PropertiesInstrument Properties Technical Data Microtome Rotary microtome Operating the instrument for the first time OperationSwitching on Switching on/fusesMains switch / automatic cutout for the instrument Automatic cutout for sectioning motorDisplay functions in control panel Control panel Key functions in control panelLock / unlock key Activates / deactivates a number of functionsY M I C R O T O M E 15-minute steps Specimen temperature Instruments with specimen cooling onlyPreset counter Instruments with sectioning motor only Cryochamber temperaturePress key until menu item ‘Preset counter’ is displayed = Preset counter activatedStarting time of automatic defrost cycle Real timeDuration of defrost cycle Press lock/unlock key to activate or de- activate ON/OFFStop Status displayThese parameters can be displayed via the ar- row keys Refrigeration stateSection thickness Total section thickness counter Instruments with sectioning motor Control panel Instruments without sectioning motorActivating a sectioning mode Sectioning mode 1 Intermittent strokeStart / stop motorized sectioning Press sectioning window key once more Emergency stop Functional area B all models Coarse feed keys Coarse feed ‘fast forward’ Travelling speed 1,000 μm/sTo start, press and hold Setting the section thickness Selecting trimming thicknessMotorized trimming ‘Defrosting Handling frozen tissue’ Chapter Daily operation of the instrument‘Knife guard / Handwheel lock’ Chapter Freezing specimens with the 90 prism Installing the 90 prismMobile heat extractor See .3.4 ‘Menu functions’ itemThermoblock Inserting specimen discs into the specimen headSpecimen orientation Manual trimming Trimming / sectioning Inserting the knifeSectioning Insert the knife / disposable blade into the knife holderSwitching off the instrument for a longer period of time Finishing work 5.6.1 Daily routine stepsLock handwheel Defrosting Automatic chamber defrost cycleInstruments with specimen cooling Manual chamber defrost cycle Troubleshooting, applications tips Remedies Potential problems causes and remedies Problem CausesProblem Causes Luke-warm specimen disc Microtome defective Problem Causes RemediesTissue Reference chart for temperature settings in minus CSafety instructions On disinfection and cleaning Disinfection, cleaning and maintenanceSpray disinfection with Leica Cryofect Allow to soak for 15 minutesSwitch the instrument off CleaningDisinfection with conventional disinfectants Remove section waste from the chamber and dispose of it‘Disinfection and cleaning’ Chapter Reinstalling the microtome Place plastic coupling 11 on handwheel couplingUnlock handwheel Exchanging the fluorescent light lamp Installing the replacement lampRemoval Removing the sliding windowWarranty Warranty and serviceTechnical service information Decommissioning and disposalElectrohydraulic height adjustment Safety instructionsAppendix Installation Maintenance and care TroubleshootingControl keys Accessories Ordering information accessories Knife holdersSpecimen discs ConsumablesDag Graupner Managing Director EC Declaration of ConformityDecontamination Certificate master This equipment has been cleaned and decontaminatedDear Customer NB Microtome knives must be in boxesBU Return Authorisation Number