Leica CM3050 S instruction manual Problem Causes

6.Troubleshooting, applications tips

Problem Causes Remedies

Sections curl on anti-roll plate.

- Anti-roll plate does not pro-	- Readjust correctly.
trude far enough beyond the
knife edge.

Scraping noise during section- ing stroke and specimen return stroke.

- Anti-roll plate protrudes too far	- Readjust correctly.
beyond the knife edge and is
scraping against the specimen.

Ondulated sections.	-	Knife damaged.	-	Use different part of the knife
				or replace knife.
	-	Edge of anti-roll plate damaged.	-	Replace anti-roll plate.

Chatter marks form during sectioning.

-Specimen insufficiently frozen onto specimen disc.

-Specimen disc not clamped tightly enough.

-Specimen holder ball joint not clamped.

-Knife not clamped tightly enough.

-Selected section thickness too thick - specimen has come off the specimen disc.

-Specimen very hard and inho- mogeneous.

-Blunt knife.

-Knife profile inappropriate for the type of specimen to be sectioned.

-Wrong clearance angle.

-Refreeze specimen onto disc.

-Check disc clamping.

-Check ball joint clamping.

-Check knife clamping.

-Refreeze specimen onto disc.

-Increase section thickness; if possible, reduce specimen surface.

-Use different part of knife or replace knife.

-Use knife with a different pro- file.

-Readjust clearance angle set- ting.

Condensation forms on anti-roll	- Brush, forceps, cloth or other	- Accessories and cleaning
plate and knife during cleaning.	cleaning item too warm.	material to be used pre-cooled
		only. - Store on storage shelves
		inside the cryochamber.

Leica CM3050 S – Cryostat 49