Leica CM3050 S instruction manual Standard delivery

Page 17

 

 

 

 

 

 

3.

Installation

 

 

 

 

 

 

 

 

 

3.3

Standard delivery

 

 

 

 

 

 

 

 

1

Cryostat with microtome

 

0460 32037

 

 

1

Handwheel with marking, antibacterial

 

0471 42558

 

 

1

Heat extractor, stationary

 

0369 11197

 

 

1

Low-temperature stabilizer for heatextractor

 

0452 27919

 

 

1

90° prism for direct specimen freezing on specimen head

 

 

 

 

with clamping screw to facilitate specimen trimming

0443 25949

 

 

1

Set of specimen discs

 

0470 43550

 

 

 

- 4 Specimen discs, 25 mm

 

0416 19275

 

 

 

- 4 Specimen discs, 30 mm

 

0370 08587

 

 

1

Storage shelf, right

 

0443 25723

 

 

1

Storage shelf, left

.........................................................................................

 

0443 33549

 

 

1

Section waste tray

 

0400 26817

 

 

1

Foot switch with protective guard

 

0502 29977

 

 

1

Rubber mat

 

 

0443 25732

 

 

1

Freezing shelf cover

 

0443 30783

 

 

1

Brush shelf

 

 

0398 13088

 

 

1

Tool set

 

 

 

0436 43463

 

 

 

-1 Brush, fine

 

 

0183 28642

 

 

 

-1 Leica-brush

 

 

0183 30751

 

 

 

-1 Allen key, size 1.5

 

0222 10050

 

 

 

-1 Allen key, size 2.5

 

0222 04137

 

 

 

-1 Allen key, size 3.0

 

0222 04138

 

 

 

-1 Allen key, size 4.0

 

0222 04139

 

 

 

-1 Allen key with spherical head, size 4.0

 

0222 32131

 

 

 

-1 Allen key, size 5.0

 

0222 04140

 

 

 

-1 Allen key with handle, size 5.0

 

0194 04760

 

 

 

-1 Allen key, size 6.0

 

0222 04141

 

 

 

-1 Single-head wrench, size 13

 

0330 33149

 

 

 

-1 Single-head wrench, size 16

 

0330 18595

 

 

1

Bottle of OCT-Compound, mounting medium

 

 

 

 

 

 

for cryosectioning, 125 ml

 

0201 08926

 

 

1

Bottle of cryostat oil, type 407, 50 ml

 

0336 06098

 

 

1

Pair of cut resistant gloves, size S

 

0340 40859

 

 

1

Instruction manual Leica CM3050 S - G/E/F/S

 

0708 37109

 

In addition to the above:

 

 

 

 

 

 

 

Instruments with specimen cooling:

Configured instruments:

Instruments with sectioning motor:

1

90° Prism

 

1

Knife holder base

1

Footswitch with

 

for low temperature quick freezing

1

Knife holder with accessories

 

protective guard

 

 

 

 

 

 

 

 

Leica CM 3050 S – Cryostat

 

 

 

 

 

17

 

Page 16 Page 18
Image 17
Page 16 Page 18
Contents Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Serial- number Symbols used in this manual and their meaningImportant information Designated use GeneralQualification of personnel Any other use of the instrument is considered improperSicherheit Integrated safety devicesLocking the handwheel SafetyLocking UnlockingKnife guard Activating the emergency stop functionDeactivating the emergency stop Electrical connections Site requirementsFrozen parts of the instrument and frozen accessories Defrosting/Handling frozen tissueHandling microtome knives/blades Knife guard/handwheel lockInfectious/radioactive material Disinfection and cleaningDisplay message ‘Dry microtome’ 11Removing/reinstalling the microtomeMaintenance Unpacking and installation InstallationGeneral site requirements RepackingStandard delivery Further accessories Inserting the accessories Models with footswitch Footswitch FunctionConnecting the footswitch Prior to switching on the instrumentOverview Instrument PropertiesInstrument Properties Technical Data Microtome Rotary microtome Operating the instrument for the first time OperationSwitching on Switching on/fusesMains switch / automatic cutout for the instrument Automatic cutout for sectioning motorDisplay functions in control panel Control panel Key functions in control panelLock / unlock key Activates / deactivates a number of functionsY M I C R O T O M E 15-minute steps Specimen temperature Instruments with specimen cooling onlyPreset counter Instruments with sectioning motor only Cryochamber temperaturePress key until menu item ‘Preset counter’ is displayed = Preset counter activatedStarting time of automatic defrost cycle Real timeDuration of defrost cycle Press lock/unlock key to activate or de- activate ON/OFFStop Status displaySection thickness Total section thickness counter These parameters can be displayed via the ar- row keysRefrigeration state Instruments with sectioning motor Control panel Instruments without sectioning motorStart / stop motorized sectioning Activating a sectioning modeSectioning mode 1 Intermittent stroke Press sectioning window key once more Emergency stop To start, press and hold Functional area B all models Coarse feed keysCoarse feed ‘fast forward’ Travelling speed 1,000 μm/s Motorized trimming Setting the section thicknessSelecting trimming thickness ‘Knife guard / Handwheel lock’ Chapter ‘Defrosting Handling frozen tissue’ ChapterDaily operation of the instrument Freezing specimens with the 90 prism Installing the 90 prismMobile heat extractor See .3.4 ‘Menu functions’ itemSpecimen orientation ThermoblockInserting specimen discs into the specimen head Manual trimming Trimming / sectioning Inserting the knifeSectioning Insert the knife / disposable blade into the knife holderLock handwheel Switching off the instrument for a longer period of timeFinishing work 5.6.1 Daily routine steps Instruments with specimen cooling DefrostingAutomatic chamber defrost cycle Manual chamber defrost cycle Troubleshooting, applications tips Remedies Potential problems causes and remedies Problem CausesProblem Causes Luke-warm specimen disc Microtome defective Problem Causes RemediesTissue Reference chart for temperature settings in minus CSafety instructions On disinfection and cleaning Disinfection, cleaning and maintenanceSpray disinfection with Leica Cryofect Allow to soak for 15 minutesSwitch the instrument off CleaningDisinfection with conventional disinfectants Remove section waste from the chamber and dispose of it‘Disinfection and cleaning’ Chapter Unlock handwheel Reinstalling the microtomePlace plastic coupling 11 on handwheel coupling Exchanging the fluorescent light lamp Installing the replacement lampRemoval Removing the sliding windowWarranty Warranty and serviceTechnical service information Decommissioning and disposalAppendix Electrohydraulic height adjustmentSafety instructions Installation Control keys Maintenance and careTroubleshooting Accessories Ordering information accessories Knife holdersSpecimen discs ConsumablesDag Graupner Managing Director EC Declaration of ConformityDecontamination Certificate master This equipment has been cleaned and decontaminatedDear Customer NB Microtome knives must be in boxesBU Return Authorisation Number
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