Leica CM3050 S instruction manual Emergency stop

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5. Operation

Functional areas A and C - Instruments with sectioning motor only

5.4.3 Selecting sectioning speed

Sectioning speed is selected via the sliding potentiometer. Speed can be changed while sectioning is in motion.

Caution when working in continuous mode:

If the slider is set to ‘0’, the sectioning motion stops, however, the actual sectioning mode is still active - just operating at ‘0’ speed.

If the slider is accidentally moved, sectioning will be resumed! - Risk of injury!

Therefore:

Do not interrupt sectioning / trim- ming by setting the sliding potenti- ometer to zero speed!

See also safety instruction in Chap- ter 2.3.6 ‘Motorized sectioning’.

Vmax key

As long as the button is pressed, the sectioning motion is carried out at maxi- mum speed (210 mm/s).

While Vmax is active, the sectioning window is inactive.

5.4.4 Emergency stop

Activate by:

either pressing the emergency stop button forcefully (must lock in position),

or depressing the footswitch force-

fully.

Either way, sectioning is interrupted at once.

In both cases, the red LED in functional area A lights up, indicating that the emergency stop function has been activated. Simultaneously all 3 sectioning modes are deactivated (= all 3 LEDs off).

To deactivate the emergency stop function, rotate the button in the direction of the ar- row until it unlocks and slips upward into its original position.

As soon as the emergency stop button has been

unlocked, the red LED is extinguished.

If the emergency stop function has been ac- tivated with the footswitch, unlocking is not necessary.

As soon as the footswitch is released, the red LED is extinguished.

To continue work, select one of sectioning modes 1 - 3 and restart the sectioning motion.

When the Vmax key is released, the sectioning window becomes active again.

See also chapter 2.2.3 ‘Safety - Emergency stop function’ for further information on the emer- gency stop.

Leica CM 3050 S – Cryostat

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Contents Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Important information Symbols used in this manual and their meaningSerial- number Designated use GeneralQualification of personnel Any other use of the instrument is considered improperSicherheit Integrated safety devicesLocking the handwheel SafetyLocking UnlockingDeactivating the emergency stop Activating the emergency stop functionKnife guard Electrical connections Site requirementsFrozen parts of the instrument and frozen accessories Defrosting/Handling frozen tissueHandling microtome knives/blades Knife guard/handwheel lockInfectious/radioactive material Disinfection and cleaningMaintenance 11Removing/reinstalling the microtomeDisplay message ‘Dry microtome’ Unpacking and installation InstallationGeneral site requirements RepackingStandard delivery Further accessories Inserting the accessories Models with footswitch Footswitch FunctionConnecting the footswitch Prior to switching on the instrumentOverview Instrument PropertiesInstrument Properties Technical Data Microtome Rotary microtome Operating the instrument for the first time OperationSwitching on Switching on/fusesMains switch / automatic cutout for the instrument Automatic cutout for sectioning motorDisplay functions in control panel Control panel Key functions in control panelLock / unlock key Activates / deactivates a number of functionsY M I C R O T O M E 15-minute steps Specimen temperature Instruments with specimen cooling onlyPreset counter Instruments with sectioning motor only Cryochamber temperaturePress key until menu item ‘Preset counter’ is displayed = Preset counter activatedStarting time of automatic defrost cycle Real timeDuration of defrost cycle Press lock/unlock key to activate or de- activate ON/OFFStop Status displayRefrigeration state These parameters can be displayed via the ar- row keysSection thickness Total section thickness counter Instruments with sectioning motor Control panel Instruments without sectioning motorSectioning mode 1 Intermittent stroke Activating a sectioning modeStart / stop motorized sectioning Press sectioning window key once more Emergency stop Coarse feed ‘fast forward’ Travelling speed 1,000 μm/s Functional area B all models Coarse feed keysTo start, press and hold Selecting trimming thickness Setting the section thicknessMotorized trimming Daily operation of the instrument ‘Defrosting Handling frozen tissue’ Chapter‘Knife guard / Handwheel lock’ Chapter Freezing specimens with the 90 prism Installing the 90 prismMobile heat extractor See .3.4 ‘Menu functions’ itemInserting specimen discs into the specimen head ThermoblockSpecimen orientation Manual trimming Trimming / sectioning Inserting the knifeSectioning Insert the knife / disposable blade into the knife holderFinishing work 5.6.1 Daily routine steps Switching off the instrument for a longer period of timeLock handwheel Automatic chamber defrost cycle DefrostingInstruments with specimen cooling Manual chamber defrost cycle Troubleshooting, applications tips Remedies Potential problems causes and remedies Problem CausesProblem Causes Luke-warm specimen disc Microtome defective Problem Causes RemediesTissue Reference chart for temperature settings in minus CSafety instructions On disinfection and cleaning Disinfection, cleaning and maintenanceSpray disinfection with Leica Cryofect Allow to soak for 15 minutesSwitch the instrument off CleaningDisinfection with conventional disinfectants Remove section waste from the chamber and dispose of it‘Disinfection and cleaning’ Chapter Place plastic coupling 11 on handwheel coupling Reinstalling the microtomeUnlock handwheel Exchanging the fluorescent light lamp Installing the replacement lampRemoval Removing the sliding windowWarranty Warranty and serviceTechnical service information Decommissioning and disposalSafety instructions Electrohydraulic height adjustmentAppendix Installation Troubleshooting Maintenance and careControl keys Accessories Ordering information accessories Knife holdersSpecimen discs ConsumablesDag Graupner Managing Director EC Declaration of ConformityDecontamination Certificate master This equipment has been cleaned and decontaminatedDear Customer NB Microtome knives must be in boxesBU Return Authorisation Number