Leica Expert Troubleshooting for Cryostat and Microtome Devices

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6.Troubleshooting, applications tips

6.1 Displayed error messages

The autodiagnosis function of the CM3050 S displays error messages in case of certain mal- functions.

1. Power failure

This error message is displayed after the instru- ment had been disconnected from mains, either due to power failure or because the mains switch had been turned off.

C

T

-

3

0

°

C

 

 

O

T

-

3

5

°

C

 

 

P

O

W

E

R

 

F

A

I

L

U

R

E

 

To acknowledge the message, press for about 5 seconds.

2. Coarse feed

Coarse feed malfunction has occurred.

C

T

-

3

0

°

C

 

 

O

T

-

3

5

°

C

 

 

 

?

?

?

?

 

 

 

 

 

 

 

 

 

This message is also displayed, if the coarse feed plug has not been reconnected properly after reinstallation of the microtome.

3. Temperature indications

C T - # # ° C

O T - 3 5 ° C

or

C

T

-

3

0

°

C

 

 

O

T

-

#

#

°

C

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

If the # symbol is displayed instead of chamber or specimen temperature indication, the corre- sponding temperature sensor is broken or a short circuit has occurred.

4. Service

C T - 3 0 ° C

O T - 3 5 ° C

S E R V I C E

This message is displayed after a certain number of operating hours to remind the user to have a preven- tive maintenance done by Leica Technical Service.

This message is displayed only, if the instrument is not regularly ser- viced by Leica Technical Service.

We recommend a maintenance contract once the warranty period has expired!

Contact your local Leica sales office for details!

If one of the error messages listed under 2, 3 and 4 is displayed, please contact Leica Technical Service.

6.1.1 Other error indications

(red ) in control panel 2 is illuminated:

Emergency stop button accidentally activat- ed.

--> Unlock.

Footswitch not properly connected.

--> Check connection - connect properly, if applicable.

Leica CM 3050 S – Cryostat

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Contents Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Serial- number Symbols used in this manual and their meaningImportant information Any other use of the instrument is considered improper GeneralDesignated use Qualification of personnelSicherheit Integrated safety devicesUnlocking SafetyLocking the handwheel LockingKnife guard Activating the emergency stop functionDeactivating the emergency stop Electrical connections Site requirementsKnife guard/handwheel lock Defrosting/Handling frozen tissueFrozen parts of the instrument and frozen accessories Handling microtome knives/bladesInfectious/radioactive material Disinfection and cleaningDisplay message ‘Dry microtome’ 11Removing/reinstalling the microtomeMaintenance Repacking InstallationUnpacking and installation General site requirementsStandard delivery Further accessories Inserting the accessories Prior to switching on the instrument Footswitch FunctionModels with footswitch Connecting the footswitchOverview Instrument PropertiesInstrument Properties Technical Data Microtome Rotary microtome Operating the instrument for the first time OperationAutomatic cutout for sectioning motor Switching on/fusesSwitching on Mains switch / automatic cutout for the instrumentActivates / deactivates a number of functions Control panel Key functions in control panelDisplay functions in control panel Lock / unlock keyY M I C R O T O M E 15-minute steps Specimen temperature Instruments with specimen cooling only= Preset counter activated Cryochamber temperaturePreset counter Instruments with sectioning motor only Press key until menu item ‘Preset counter’ is displayedPress lock/unlock key to activate or de- activate ON/OFF Real timeStarting time of automatic defrost cycle Duration of defrost cycleStop Status displaySection thickness Total section thickness counter These parameters can be displayed via the ar- row keysRefrigeration state Instruments with sectioning motor Control panel Instruments without sectioning motorStart / stop motorized sectioning Activating a sectioning modeSectioning mode 1 Intermittent stroke Press sectioning window key once more Emergency stop To start, press and hold Functional area B all models Coarse feed keysCoarse feed ‘fast forward’ Travelling speed 1,000 μm/s Motorized trimming Setting the section thicknessSelecting trimming thickness ‘Knife guard / Handwheel lock’ Chapter ‘Defrosting Handling frozen tissue’ ChapterDaily operation of the instrument See .3.4 ‘Menu functions’ item Installing the 90 prismFreezing specimens with the 90 prism Mobile heat extractorSpecimen orientation ThermoblockInserting specimen discs into the specimen head Insert the knife / disposable blade into the knife holder Trimming / sectioning Inserting the knifeManual trimming SectioningLock handwheel Switching off the instrument for a longer period of timeFinishing work 5.6.1 Daily routine steps Instruments with specimen cooling DefrostingAutomatic chamber defrost cycle Manual chamber defrost cycle Troubleshooting, applications tips Remedies Potential problems causes and remedies Problem CausesProblem Causes Luke-warm specimen disc Microtome defective Problem Causes RemediesTissue Reference chart for temperature settings in minus CAllow to soak for 15 minutes Disinfection, cleaning and maintenanceSafety instructions On disinfection and cleaning Spray disinfection with Leica CryofectRemove section waste from the chamber and dispose of it CleaningSwitch the instrument off Disinfection with conventional disinfectants‘Disinfection and cleaning’ Chapter Unlock handwheel Reinstalling the microtomePlace plastic coupling 11 on handwheel coupling Removing the sliding window Installing the replacement lampExchanging the fluorescent light lamp RemovalDecommissioning and disposal Warranty and serviceWarranty Technical service informationAppendix Electrohydraulic height adjustmentSafety instructions Installation Control keys Maintenance and careTroubleshooting Consumables Ordering information accessories Knife holdersAccessories Specimen discsDag Graupner Managing Director EC Declaration of ConformityNB Microtome knives must be in boxes This equipment has been cleaned and decontaminatedDecontamination Certificate master Dear CustomerBU Return Authorisation Number