Leica CM3050 S instruction manual General, Designated use, Qualification of personnel

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1.Important information

General

This instruction manual includes important instructions and information related to the operating safety and maintenance of the instrument.

The instruction manual is an important part of the product. It must be read carefully before using the instrument for the first time and must always be kept with the instrument.

If additional requirements, which exceed the scope of this manual, are imposed by regula-tions and/or laws on accident prevention and environmental protection in the country of operation, appropriate instructions for compliance with such requirements must be added to this manual.

Read this instruction manual carefully before attempting to use or operate the instrument.

Please pay particular attention to chapter 2 (safety features, safety instructions). - Please read this information, even if you are already familiar with the operation and use of other Leica products.

1.2Designated use

The Leica CM3050S is a powerful cryostat for routine as well as research applications in biology, medicine and industry.

The instrument has been designed for rapid freezing and sectioning of tissue samples.

The instrument has not been designed for un- attended storage of tissue material.

The instrument may only be operated within the scope of its designated use as described above and as per the instructions given in this manual.

Any other use of the instrument is considered improper.

1.3Qualification of personnel

The Leica CM3050S may only be operated by trained laboratory personnel.

All laboratory personnel designated to operate the instrument must carefully read the present instruction manual prior to starting work with the instrument.

8	Instruction Manual V1.2 - 11/2006

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Contents Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Serial- number Symbols used in this manual and their meaningImportant information General Designated useQualification of personnel Any other use of the instrument is considered improperIntegrated safety devices SicherheitSafety Locking the handwheelLocking UnlockingKnife guard Activating the emergency stop functionDeactivating the emergency stop Site requirements Electrical connectionsDefrosting/Handling frozen tissue Frozen parts of the instrument and frozen accessoriesHandling microtome knives/blades Knife guard/handwheel lockDisinfection and cleaning Infectious/radioactive materialDisplay message ‘Dry microtome’ 11Removing/reinstalling the microtomeMaintenance Installation Unpacking and installationGeneral site requirements RepackingStandard delivery Further accessories Inserting the accessories Footswitch Function Models with footswitchConnecting the footswitch Prior to switching on the instrumentInstrument Properties OverviewInstrument Properties Technical Data Microtome Rotary microtome Operation Operating the instrument for the first timeSwitching on/fuses Switching onMains switch / automatic cutout for the instrument Automatic cutout for sectioning motorControl panel Key functions in control panel Display functions in control panelLock / unlock key Activates / deactivates a number of functionsY M I C R O T O M E Specimen temperature Instruments with specimen cooling only 15-minute stepsCryochamber temperature Preset counter Instruments with sectioning motor onlyPress key until menu item ‘Preset counter’ is displayed = Preset counter activatedReal time Starting time of automatic defrost cycleDuration of defrost cycle Press lock/unlock key to activate or de- activate ON/OFFStatus display StopSection thickness Total section thickness counter These parameters can be displayed via the ar- row keysRefrigeration state Control panel Instruments without sectioning motor Instruments with sectioning motorStart / stop motorized sectioning Activating a sectioning modeSectioning mode 1 Intermittent stroke Press sectioning window key once more Emergency stop To start, press and hold Functional area B all models Coarse feed keysCoarse feed ‘fast forward’ Travelling speed 1,000 μm/s Motorized trimming Setting the section thicknessSelecting trimming thickness ‘Knife guard / Handwheel lock’ Chapter ‘Defrosting Handling frozen tissue’ ChapterDaily operation of the instrument Installing the 90 prism Freezing specimens with the 90 prismMobile heat extractor See .3.4 ‘Menu functions’ itemSpecimen orientation ThermoblockInserting specimen discs into the specimen head Trimming / sectioning Inserting the knife Manual trimmingSectioning Insert the knife / disposable blade into the knife holderLock handwheel Switching off the instrument for a longer period of timeFinishing work 5.6.1 Daily routine steps Instruments with specimen cooling DefrostingAutomatic chamber defrost cycle Manual chamber defrost cycle Troubleshooting, applications tips Potential problems causes and remedies Problem Causes RemediesProblem Causes Luke-warm specimen disc Problem Causes Remedies Microtome defectiveReference chart for temperature settings in minus C TissueDisinfection, cleaning and maintenance Safety instructions On disinfection and cleaningSpray disinfection with Leica Cryofect Allow to soak for 15 minutesCleaning Switch the instrument offDisinfection with conventional disinfectants Remove section waste from the chamber and dispose of it‘Disinfection and cleaning’ Chapter Unlock handwheel Reinstalling the microtomePlace plastic coupling 11 on handwheel coupling Installing the replacement lamp Exchanging the fluorescent light lampRemoval Removing the sliding windowWarranty and service WarrantyTechnical service information Decommissioning and disposalAppendix Electrohydraulic height adjustmentSafety instructions Installation Control keys Maintenance and careTroubleshooting Ordering information accessories Knife holders AccessoriesSpecimen discs ConsumablesEC Declaration of Conformity Dag Graupner Managing DirectorThis equipment has been cleaned and decontaminated Decontamination Certificate masterDear Customer NB Microtome knives must be in boxesBU Return Authorisation Number