Leica CM3050 S instruction manual Activating a sectioning mode, Start / stop motorized sectioning

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5. Operation

Functional areas A and C - Instruments with sectioning motor only

5.4.1Selecting a sectioning mode / start and stop sectioning

Three different sectioning modes can be select- ed for motorized sectioning:

Sectioning mode 1 - Intermittent stroke: The specimen head travels up and down as long as the RUN/STOP and RUN/ENABLE keys are pressed simulta- neously or as long as the footswitch is depressed.

Sectioning mode 2 - Continuous stroke: The specimen head travels up and down until it is stopped by pressing RUN/STOP or RUN/ENABLE or by depressing the footswitch.

Sectioning mode 3 - Single stroke:

The specimen head carries out one sec- tioning stroke and then stops in the low- er limit position.

Activating a sectioning mode

Press one of the three sectioning mode keys de- scribed above. While a sectioning mode is ac- tive, the LED in the corresponding key will be il- luminated.

Start / stop motorized sectioning

Sectioning mode 1 - Intermittent stroke:

•Simultaneously press and hold RUN/STOP and RUN/ENABLE or depress and hold footswitch.

•To stop the sectioning stroke, release the keys or the footswitch:

The specimen head stops immediately.

Sectioning mode 2 - Continuous stroke:

•Simultaneously press and release RUN/STOP and RUN/ENABLE or depress and release footswitch.

•To stop sectioning:

Press and release RUN/STOP or RUN/EN- ABLE or depress and release the footswitch:

The specimen head stops upon reaching the lower limit position.

Sectioning mode 3 - Single stroke:

•Simultaneously press and release RUN/STOP and RUN/ENABLE or depress and release footswitch.

•The instrument carries out one complete section / retraction stroke and the specimen head stops upon reaching the lower limit po- sition.

While sectioning is in motion, LED ‘RUN’ is illu- minated.

While the sectioning motor is deactivated, LED ‘STOP’ is illuminated.

Both LEDs are located in functional area C.

Leica CM 3050 S – Cryostat

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Contents Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Serial- number Symbols used in this manual and their meaningImportant information Any other use of the instrument is considered improper GeneralDesignated use Qualification of personnelSicherheit Integrated safety devicesUnlocking SafetyLocking the handwheel LockingKnife guard Activating the emergency stop functionDeactivating the emergency stop Electrical connections Site requirementsKnife guard/handwheel lock Defrosting/Handling frozen tissueFrozen parts of the instrument and frozen accessories Handling microtome knives/bladesInfectious/radioactive material Disinfection and cleaningDisplay message ‘Dry microtome’ 11Removing/reinstalling the microtomeMaintenance Repacking InstallationUnpacking and installation General site requirementsStandard delivery Further accessories Inserting the accessories Prior to switching on the instrument Footswitch FunctionModels with footswitch Connecting the footswitchOverview Instrument PropertiesInstrument Properties Technical Data Microtome Rotary microtome Operating the instrument for the first time OperationAutomatic cutout for sectioning motor Switching on/fusesSwitching on Mains switch / automatic cutout for the instrumentActivates / deactivates a number of functions Control panel Key functions in control panelDisplay functions in control panel Lock / unlock keyY M I C R O T O M E 15-minute steps Specimen temperature Instruments with specimen cooling only= Preset counter activated Cryochamber temperaturePreset counter Instruments with sectioning motor only Press key until menu item ‘Preset counter’ is displayedPress lock/unlock key to activate or de- activate ON/OFF Real timeStarting time of automatic defrost cycle Duration of defrost cycleStop Status displaySection thickness Total section thickness counter These parameters can be displayed via the ar- row keysRefrigeration state Instruments with sectioning motor Control panel Instruments without sectioning motorStart / stop motorized sectioning Activating a sectioning modeSectioning mode 1 Intermittent stroke Press sectioning window key once more Emergency stop To start, press and hold Functional area B all models Coarse feed keysCoarse feed ‘fast forward’ Travelling speed 1,000 μm/s Motorized trimming Setting the section thicknessSelecting trimming thickness ‘Knife guard / Handwheel lock’ Chapter ‘Defrosting Handling frozen tissue’ ChapterDaily operation of the instrument See .3.4 ‘Menu functions’ item Installing the 90 prismFreezing specimens with the 90 prism Mobile heat extractorSpecimen orientation ThermoblockInserting specimen discs into the specimen head Insert the knife / disposable blade into the knife holder Trimming / sectioning Inserting the knifeManual trimming SectioningLock handwheel Switching off the instrument for a longer period of timeFinishing work 5.6.1 Daily routine steps Instruments with specimen cooling DefrostingAutomatic chamber defrost cycle Manual chamber defrost cycle Troubleshooting, applications tips Remedies Potential problems causes and remedies Problem CausesProblem Causes Luke-warm specimen disc Microtome defective Problem Causes RemediesTissue Reference chart for temperature settings in minus CAllow to soak for 15 minutes Disinfection, cleaning and maintenanceSafety instructions On disinfection and cleaning Spray disinfection with Leica CryofectRemove section waste from the chamber and dispose of it CleaningSwitch the instrument off Disinfection with conventional disinfectants‘Disinfection and cleaning’ Chapter Unlock handwheel Reinstalling the microtomePlace plastic coupling 11 on handwheel coupling Removing the sliding window Installing the replacement lampExchanging the fluorescent light lamp RemovalDecommissioning and disposal Warranty and serviceWarranty Technical service informationAppendix Electrohydraulic height adjustmentSafety instructions Installation Control keys Maintenance and careTroubleshooting Consumables Ordering information accessories Knife holdersAccessories Specimen discsDag Graupner Managing Director EC Declaration of ConformityNB Microtome knives must be in boxes This equipment has been cleaned and decontaminatedDecontamination Certificate master Dear CustomerBU Return Authorisation Number