Leica CM3050 S instruction manual Press sectioning window key once more

Page 36

5. Operation

Functional areas A and C - Instruments with sectioning motor only

5.4.2 Setting the sectioning window

The term ‘Sectioning window’ stands for a dif- ferent (slower) speed setting in that part of the sectioning stroke where the specimen is actual- ly cut, while the remaining sectioning stroke (above and below the specimen) as well as the retraction (return) stroke are carried out at a higher speed level (time-saving feature).

If a sectioning window is set, speed inside the window is as set on the control panel. Outside the sectioning window the specimen head trav- els at a proportionately higher speed.

Always set the sectioning window while in the sectioning stroke (speci- men moves downward). Setting the window in retraction stroke (speci- men travels upward) is not possible.

Position lower edge of specimen approx. 3 mm above the knife edge (rotate hand- wheel clockwise).

Press sectioning window key.

Position upper edge of specimen just below the knife edge (continue to rotate the hand- wheel clockwise).

The value has been stored when the LED in the sectioning window key lights up briefly.

If the LED flashes, the value has not yet been stored, because the user has tried to set a win- dow margin outside the possible window range (the area in which the sectioning window can be set is slightly smaller than the total section stroke).

In that case, repeat the step in question.

.

The sectioning window cannot be deleted. If need be, it can be set to maximum size (i.e. almost the en- tire sectioning stroke will be car- ried out at one and the same speed).

Every time after the instrument is disconnected from mains, the sec- tioning window is set to maximum size upon switching back on. This also applies when the instrument is switched on for the first time.

Press sectioning window key once more.

36

Instruction Manual V1.2 - 11/2006

Image 36
Contents Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Symbols used in this manual and their meaning Important informationSerial- number General Designated useQualification of personnel Any other use of the instrument is considered improperIntegrated safety devices SicherheitSafety Locking the handwheelLocking UnlockingActivating the emergency stop function Deactivating the emergency stopKnife guard Site requirements Electrical connectionsDefrosting/Handling frozen tissue Frozen parts of the instrument and frozen accessoriesHandling microtome knives/blades Knife guard/handwheel lockDisinfection and cleaning Infectious/radioactive material11Removing/reinstalling the microtome MaintenanceDisplay message ‘Dry microtome’ Installation Unpacking and installationGeneral site requirements RepackingStandard delivery Further accessories Inserting the accessories Footswitch Function Models with footswitchConnecting the footswitch Prior to switching on the instrumentInstrument Properties OverviewInstrument Properties Technical Data Microtome Rotary microtome Operation Operating the instrument for the first timeSwitching on/fuses Switching onMains switch / automatic cutout for the instrument Automatic cutout for sectioning motorControl panel Key functions in control panel Display functions in control panelLock / unlock key Activates / deactivates a number of functionsY M I C R O T O M E Specimen temperature Instruments with specimen cooling only 15-minute stepsCryochamber temperature Preset counter Instruments with sectioning motor onlyPress key until menu item ‘Preset counter’ is displayed = Preset counter activatedReal time Starting time of automatic defrost cycleDuration of defrost cycle Press lock/unlock key to activate or de- activate ON/OFFStatus display StopThese parameters can be displayed via the ar- row keys Refrigeration stateSection thickness Total section thickness counter Control panel Instruments without sectioning motor Instruments with sectioning motorActivating a sectioning mode Sectioning mode 1 Intermittent strokeStart / stop motorized sectioning Press sectioning window key once more Emergency stop Functional area B all models Coarse feed keys Coarse feed ‘fast forward’ Travelling speed 1,000 μm/sTo start, press and hold Setting the section thickness Selecting trimming thicknessMotorized trimming ‘Defrosting Handling frozen tissue’ Chapter Daily operation of the instrument‘Knife guard / Handwheel lock’ Chapter Installing the 90 prism Freezing specimens with the 90 prismMobile heat extractor See .3.4 ‘Menu functions’ itemThermoblock Inserting specimen discs into the specimen headSpecimen orientation Trimming / sectioning Inserting the knife Manual trimmingSectioning Insert the knife / disposable blade into the knife holderSwitching off the instrument for a longer period of time Finishing work 5.6.1 Daily routine stepsLock handwheel Defrosting Automatic chamber defrost cycleInstruments with specimen cooling Manual chamber defrost cycle Troubleshooting, applications tips Potential problems causes and remedies Problem Causes RemediesProblem Causes Luke-warm specimen disc Problem Causes Remedies Microtome defectiveReference chart for temperature settings in minus C TissueDisinfection, cleaning and maintenance Safety instructions On disinfection and cleaningSpray disinfection with Leica Cryofect Allow to soak for 15 minutesCleaning Switch the instrument offDisinfection with conventional disinfectants Remove section waste from the chamber and dispose of it‘Disinfection and cleaning’ Chapter Reinstalling the microtome Place plastic coupling 11 on handwheel couplingUnlock handwheel Installing the replacement lamp Exchanging the fluorescent light lampRemoval Removing the sliding windowWarranty and service WarrantyTechnical service information Decommissioning and disposalElectrohydraulic height adjustment Safety instructionsAppendix Installation Maintenance and care TroubleshootingControl keys Ordering information accessories Knife holders AccessoriesSpecimen discs ConsumablesEC Declaration of Conformity Dag Graupner Managing DirectorThis equipment has been cleaned and decontaminated Decontamination Certificate masterDear Customer NB Microtome knives must be in boxesBU Return Authorisation Number