Leica CM3050 S Selecting trimming thickness, Motorized trimming, Setting the section thickness

Page 39

5.Operation

Functional area B - all models

5.4.6Trimming and sectioning functions Switching between trimming and sectioning

Press this key to alternate between the two functions.

illuminated:

Trimming mode is active

off:

Sectioning mode is active.

Selecting trimming thickness

Activate trimming mode

Select trimming thickness (= sectioning thickness at which trimming is carried out) by pressing or .

The selected thickness is displayed in the cor-

responding LED :

Motorized trimming

Select ‘Continuous stroke’ in functional area A.

Activate trimming by pressing RUN/STOP and RUN/ENABLE (located in functional area C) simultaneously or by depressing the footswitch.

To stop trimming press RUN/STOP or RUN/ ENABLE or depress the footswitch again.

Trimming thickness can be changed while trim- ming is in motion by pressing or .

5.4.7 Setting the section thickness

• Activate sectioning mode ( off).

C T . . . . ° C O T . . . . ° C

μ 0 1 0 . 0

Press or to select section thickness. Pressing one of these keys once makes the display in control panel 1 switch to section thickness indication (thickness value not yet

being altered). Pressing or further starts to change the actual section thick- ness setting.

Selectable section thickness settings:

0

-

2

μm

in 0.5-μm

steps,

2

-

10

μm

in

1-μm

steps,

10

-

20

μm

in

2-μm

steps,

20

-

60

μm

in

5-μm

steps,

60

-

100

μm

in 10-μm

steps,

100

-

300

μm

in 50-μm

steps.

Leica CM 3050 S – Cryostat

39

Image 39
Contents Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Symbols used in this manual and their meaning Important informationSerial- number Any other use of the instrument is considered improper GeneralDesignated use Qualification of personnelSicherheit Integrated safety devicesUnlocking SafetyLocking the handwheel LockingActivating the emergency stop function Deactivating the emergency stopKnife guard Electrical connections Site requirementsKnife guard/handwheel lock Defrosting/Handling frozen tissueFrozen parts of the instrument and frozen accessories Handling microtome knives/bladesInfectious/radioactive material Disinfection and cleaning11Removing/reinstalling the microtome MaintenanceDisplay message ‘Dry microtome’ Repacking InstallationUnpacking and installation General site requirementsStandard delivery Further accessories Inserting the accessories Prior to switching on the instrument Footswitch FunctionModels with footswitch Connecting the footswitchOverview Instrument PropertiesInstrument Properties Technical Data Microtome Rotary microtome Operating the instrument for the first time OperationAutomatic cutout for sectioning motor Switching on/fusesSwitching on Mains switch / automatic cutout for the instrumentActivates / deactivates a number of functions Control panel Key functions in control panelDisplay functions in control panel Lock / unlock keyY M I C R O T O M E 15-minute steps Specimen temperature Instruments with specimen cooling only= Preset counter activated Cryochamber temperaturePreset counter Instruments with sectioning motor only Press key until menu item ‘Preset counter’ is displayedPress lock/unlock key to activate or de- activate ON/OFF Real timeStarting time of automatic defrost cycle Duration of defrost cycleStop Status displayThese parameters can be displayed via the ar- row keys Refrigeration stateSection thickness Total section thickness counter Instruments with sectioning motor Control panel Instruments without sectioning motorActivating a sectioning mode Sectioning mode 1 Intermittent strokeStart / stop motorized sectioning Press sectioning window key once more Emergency stop Functional area B all models Coarse feed keys Coarse feed ‘fast forward’ Travelling speed 1,000 μm/sTo start, press and hold Setting the section thickness Selecting trimming thicknessMotorized trimming ‘Defrosting Handling frozen tissue’ Chapter Daily operation of the instrument‘Knife guard / Handwheel lock’ Chapter See .3.4 ‘Menu functions’ item Installing the 90 prismFreezing specimens with the 90 prism Mobile heat extractorThermoblock Inserting specimen discs into the specimen headSpecimen orientation Insert the knife / disposable blade into the knife holder Trimming / sectioning Inserting the knifeManual trimming SectioningSwitching off the instrument for a longer period of time Finishing work 5.6.1 Daily routine stepsLock handwheel Defrosting Automatic chamber defrost cycleInstruments with specimen cooling Manual chamber defrost cycle Troubleshooting, applications tips Remedies Potential problems causes and remedies Problem CausesProblem Causes Luke-warm specimen disc Microtome defective Problem Causes RemediesTissue Reference chart for temperature settings in minus CAllow to soak for 15 minutes Disinfection, cleaning and maintenanceSafety instructions On disinfection and cleaning Spray disinfection with Leica CryofectRemove section waste from the chamber and dispose of it CleaningSwitch the instrument off Disinfection with conventional disinfectants‘Disinfection and cleaning’ Chapter Reinstalling the microtome Place plastic coupling 11 on handwheel couplingUnlock handwheel Removing the sliding window Installing the replacement lampExchanging the fluorescent light lamp RemovalDecommissioning and disposal Warranty and serviceWarranty Technical service informationElectrohydraulic height adjustment Safety instructionsAppendix Installation Maintenance and care TroubleshootingControl keys Consumables Ordering information accessories Knife holdersAccessories Specimen discsDag Graupner Managing Director EC Declaration of ConformityNB Microtome knives must be in boxes This equipment has been cleaned and decontaminatedDecontamination Certificate master Dear CustomerBU Return Authorisation Number