Leica Complete Guide to CM3050 S Manual and Cryostat Operations

5.Operation

4.Quick-freezing at maximum specimen temperature* (instruments with specimen cooling only)

Press key until menu item ‘Fast object freezing’ is displayed:

F

A

S

T

O

B

J

F

R

E

E

Z

I

N

O

F

F

or

F A S T O B J F R E E Z I N

O N

Press lock/unlock key to activate or de- activate (ON/OFF).

5. Real time

Press key until menu item ‘Time’ is dis- played:

T I M E 1 0 : 2 3

•Use arrow keys ( or ) to set real time. Setting minutes as well as hours in 1-minute steps. Holding arrow button in- creases scrolling speed!

6. Starting time of automatic defrost cycle:

Press key until menu item ‘Defrost time’ is displayed:

D E F R T I M E 4 : 3 0

(Time on display = start time of defrost cycle)

•Use arrow keys ( or ) to select the de- sired defrost start time.

*) with 90° prism - see also chapter 5.5.2.

The automatic defrost cycle takes place once every 24 hours at the selected defrost time.

7. Duration of defrost cycle:

Press key until menu item ‘Defrost period’ is displayed:

D

E

F

R

O

S

T

P

E

R

I

O

D

C

H

A

M

B

E

R

:

0

8

M

I

N

•Use defrost keys ( or ) to adjust de- frost time. (Only if necessary - see below).

Important information for setting the defrost parameters

Starting time of defrost cycle:

We recommend to program the automatic defrost cycle for a time out- side regular working hours as a period of 4 hours should pass after the defrost cycle before work with the instrument is resumed (see also chapter 5.7.1 ‘Automatic chamber defrost cycle’).

Defrost cycle duration:

The duration of the chamber defrost cycle is set to 8 minutes in factory. This setting should only be changed, if the evaporator is not completely de-iced during the 8-minute cycle.

In such cases, increase the defrost cycle duration gradually until the the evaporator is ice-free after defrosting. The selected defrost cycle duration is valid for both automatic and manual chamber defrost cycle.

Leica CM3050 S – Cryostat 31