Leica Comprehensive Guide to the Properties of 's Projection Television Models

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4. Instrument Properties

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22	Instruction Manual V1.2 - 11/2006

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Contents Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Important information Symbols used in this manual and their meaningSerial- number Qualification of personnel GeneralDesignated use Any other use of the instrument is considered improperIntegrated safety devices SicherheitLocking SafetyLocking the handwheel UnlockingDeactivating the emergency stop Activating the emergency stop functionKnife guard Site requirements Electrical connectionsHandling microtome knives/blades Defrosting/Handling frozen tissueFrozen parts of the instrument and frozen accessories Knife guard/handwheel lockDisinfection and cleaning Infectious/radioactive materialMaintenance 11Removing/reinstalling the microtomeDisplay message ‘Dry microtome’ General site requirements InstallationUnpacking and installation RepackingStandard delivery Further accessories Inserting the accessories Connecting the footswitch Footswitch FunctionModels with footswitch Prior to switching on the instrumentInstrument Properties OverviewInstrument Properties Technical Data Microtome Rotary microtome Operation Operating the instrument for the first timeMains switch / automatic cutout for the instrument Switching on/fusesSwitching on Automatic cutout for sectioning motorLock / unlock key Control panel Key functions in control panelDisplay functions in control panel Activates / deactivates a number of functionsY M I C R O T O M E Specimen temperature Instruments with specimen cooling only 15-minute stepsPress key until menu item ‘Preset counter’ is displayed Cryochamber temperaturePreset counter Instruments with sectioning motor only = Preset counter activatedDuration of defrost cycle Real timeStarting time of automatic defrost cycle Press lock/unlock key to activate or de- activate ON/OFFStatus display StopRefrigeration state These parameters can be displayed via the ar- row keysSection thickness Total section thickness counter Control panel Instruments without sectioning motor Instruments with sectioning motorSectioning mode 1 Intermittent stroke Activating a sectioning modeStart / stop motorized sectioning Press sectioning window key once more Emergency stop Coarse feed ‘fast forward’ Travelling speed 1,000 μm/s Functional area B all models Coarse feed keysTo start, press and hold Selecting trimming thickness Setting the section thicknessMotorized trimming Daily operation of the instrument ‘Defrosting Handling frozen tissue’ Chapter‘Knife guard / Handwheel lock’ Chapter Mobile heat extractor Installing the 90 prismFreezing specimens with the 90 prism See .3.4 ‘Menu functions’ itemInserting specimen discs into the specimen head ThermoblockSpecimen orientation Sectioning Trimming / sectioning Inserting the knifeManual trimming Insert the knife / disposable blade into the knife holderFinishing work 5.6.1 Daily routine steps Switching off the instrument for a longer period of timeLock handwheel Automatic chamber defrost cycle DefrostingInstruments with specimen cooling Manual chamber defrost cycle Troubleshooting, applications tips Potential problems causes and remedies Problem Causes RemediesProblem Causes Luke-warm specimen disc Problem Causes Remedies Microtome defectiveReference chart for temperature settings in minus C TissueSpray disinfection with Leica Cryofect Disinfection, cleaning and maintenanceSafety instructions On disinfection and cleaning Allow to soak for 15 minutesDisinfection with conventional disinfectants CleaningSwitch the instrument off Remove section waste from the chamber and dispose of it‘Disinfection and cleaning’ Chapter Place plastic coupling 11 on handwheel coupling Reinstalling the microtomeUnlock handwheel Removal Installing the replacement lampExchanging the fluorescent light lamp Removing the sliding windowTechnical service information Warranty and serviceWarranty Decommissioning and disposalSafety instructions Electrohydraulic height adjustmentAppendix Installation Troubleshooting Maintenance and careControl keys Specimen discs Ordering information accessories Knife holdersAccessories ConsumablesEC Declaration of Conformity Dag Graupner Managing DirectorDear Customer This equipment has been cleaned and decontaminatedDecontamination Certificate master NB Microtome knives must be in boxesBU Return Authorisation Number