Leica CM3050 S Cryochamber temperature, Preset counter Instruments with sectioning motor only

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5.Operation

2. Cryochamber temperature:

Press key until menu item ‘Set chamber temperature’ is displayed:

--> Continue as described for specimen temperature.

3.Preset counter

(instruments with sectioning motor only):

Press key until menu item ‘Preset counter’ is displayed:

P R E S E T C O U N T E R

d n - - -

= Preset counter activated

P R E S E T C O U N T E R

d n 0 5 6

= Preset counter deactivated

Preset counter function:

The preset counter allows the user to carry out a predefined number of sections in the ‘Continu- ous stroke’ sectioning mode.

Once the preselected number of sections has been carried out (= section counter reading ‘0’), the specimen head will stop automatically in the lower limit position.

The reference value (i.e. the value from which the preset counter counts downward) can be selected in the corresponding menu item.

In order to be able to set a reference value, the preset counter function must be active.

If the preset counter is deactivated (see diagrams above), pressto activate.

Now there are two options:

1.Increase or decrease the actual reference value by pressing or .

2.Press and simultaneously in order to reload the former reference value (‘0’ when setting up the instrument) and then press ei-

ther or individually in order to select the desired reference value. Depending on where you start from and where you want to go, method 1 or method 2 is quicker (see ex- amples).

Example 1:

Actual preset counter reading = 50 Former reference value = 300

New reference value of 65 to be selected. Method 1 is most convenient:

• Increase from 50 to 65 by pressing .

Example 2:

Actual preset counter reading = 50 Former reference value = 300

New reference value of 320 to be selected. Method 2 is most convenient:

Press and simultaneously to reload old reference value (300):

Increase from 300 to 320 by pressing .

For further information on the preset counter, please refer to chapter 5.3.6, Item 4 ‘Preset counter’.

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Instruction Manual V1.2 - 11/2006

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Contents Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Symbols used in this manual and their meaning Important informationSerial- number Qualification of personnel GeneralDesignated use Any other use of the instrument is considered improperIntegrated safety devices SicherheitLocking SafetyLocking the handwheel UnlockingActivating the emergency stop function Deactivating the emergency stopKnife guard Site requirements Electrical connectionsHandling microtome knives/blades Defrosting/Handling frozen tissueFrozen parts of the instrument and frozen accessories Knife guard/handwheel lockDisinfection and cleaning Infectious/radioactive material11Removing/reinstalling the microtome MaintenanceDisplay message ‘Dry microtome’ General site requirements InstallationUnpacking and installation RepackingStandard delivery Further accessories Inserting the accessories Connecting the footswitch Footswitch FunctionModels with footswitch Prior to switching on the instrumentInstrument Properties OverviewInstrument Properties Technical Data Microtome Rotary microtome Operation Operating the instrument for the first timeMains switch / automatic cutout for the instrument Switching on/fusesSwitching on Automatic cutout for sectioning motorLock / unlock key Control panel Key functions in control panelDisplay functions in control panel Activates / deactivates a number of functionsY M I C R O T O M E Specimen temperature Instruments with specimen cooling only 15-minute stepsPress key until menu item ‘Preset counter’ is displayed Cryochamber temperaturePreset counter Instruments with sectioning motor only = Preset counter activatedDuration of defrost cycle Real timeStarting time of automatic defrost cycle Press lock/unlock key to activate or de- activate ON/OFFStatus display StopThese parameters can be displayed via the ar- row keys Refrigeration stateSection thickness Total section thickness counter Control panel Instruments without sectioning motor Instruments with sectioning motorActivating a sectioning mode Sectioning mode 1 Intermittent strokeStart / stop motorized sectioning Press sectioning window key once more Emergency stop Functional area B all models Coarse feed keys Coarse feed ‘fast forward’ Travelling speed 1,000 μm/sTo start, press and hold Setting the section thickness Selecting trimming thicknessMotorized trimming ‘Defrosting Handling frozen tissue’ Chapter Daily operation of the instrument‘Knife guard / Handwheel lock’ Chapter Mobile heat extractor Installing the 90 prismFreezing specimens with the 90 prism See .3.4 ‘Menu functions’ itemThermoblock Inserting specimen discs into the specimen headSpecimen orientation Sectioning Trimming / sectioning Inserting the knifeManual trimming Insert the knife / disposable blade into the knife holderSwitching off the instrument for a longer period of time Finishing work 5.6.1 Daily routine stepsLock handwheel Defrosting Automatic chamber defrost cycleInstruments with specimen cooling Manual chamber defrost cycle Troubleshooting, applications tips Potential problems causes and remedies Problem Causes RemediesProblem Causes Luke-warm specimen disc Problem Causes Remedies Microtome defectiveReference chart for temperature settings in minus C TissueSpray disinfection with Leica Cryofect Disinfection, cleaning and maintenanceSafety instructions On disinfection and cleaning Allow to soak for 15 minutesDisinfection with conventional disinfectants CleaningSwitch the instrument off Remove section waste from the chamber and dispose of it‘Disinfection and cleaning’ Chapter Reinstalling the microtome Place plastic coupling 11 on handwheel couplingUnlock handwheel Removal Installing the replacement lampExchanging the fluorescent light lamp Removing the sliding windowTechnical service information Warranty and serviceWarranty Decommissioning and disposalElectrohydraulic height adjustment Safety instructionsAppendix Installation Maintenance and care TroubleshootingControl keys Specimen discs Ordering information accessories Knife holdersAccessories ConsumablesEC Declaration of Conformity Dag Graupner Managing DirectorDear Customer This equipment has been cleaned and decontaminatedDecontamination Certificate master NB Microtome knives must be in boxesBU Return Authorisation Number