Leica CM3050 S instruction manual Microtome Rotary microtome

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4. Instrument properties

*) Refrigerant and compressor oil to be replaced only by trained and authorized service personnel!

 

CM3050S-3

CM3050S-6

CM3050S-7

 

Microtome

 

 

 

c-UL

-

-

 

Rotary microtome

 

 

 

120 V AC ±10%

208 V AC ±10%

230 V AC ±10%

Section thickness setting

 

0.5 - 300 μm

 

60 Hz

60 Hz

60 Hz

 

Overall specimen feed

 

25 mm

 

1800 VA

1800 VA

1800 VA

 

Vertical stroke

 

59 mm

 

35 A eff.

30 A eff.

25 A eff.

 

Specimen retraction

 

50 μm

 

I

I

I

 

Maximum specimen size

 

40 x 55 mm

 

T15A T1

T12A T1

T10A T1

 

Specimen orientation

(x-, y-, z-axis)

 

2

2

2

 

Trimming (6 thickness settings)

5 - 150 μm

 

II

II

II

 

Motorized coarse feed

 

 

 

1800 J/s

1800 J/s

1800 J/s

 

 

- slow

 

500 μm/s

 

 

 

 

 

 

- fast

 

1,000 μm/s

 

Fluorescent light lamp (cryochamber illumination):

Sectioning motor (Option):

 

 

 

 

 

 

 

 

 

 

Sectioning speed

 

 

 

50 Hz version:

Osram Dulux S 11 W/21

 

 

- slow

min.

0.1 mm/s

 

 

Light color ‘LUMILUX hellweiß’

 

 

max.

100 mm/s

 

 

 

(brilliant white)

 

 

vmax

210 mm/s

 

60 Hz version:

Osram Dulux S 13 W/21

 

 

- fast

min.

0.1 mm/s

 

 

Light color ‘LUMILUX hellweiß’

 

 

max.

170 mm/s

 

 

 

(brilliant white)

 

 

vmax

210 mm/s

 

 

 

 

 

 

 

 

 

 

Cryocabinet

 

 

 

 

 

 

 

 

Dimensions

 

 

 

1

according to IEC-1010, UL 3101

 

Width (w/o handwheel)

800 mm

2

according to CECOMAF:

 

 

Width (including handwheel)

882 mm

 

 

 

Liquid temperature 45°C

 

 

Depth

 

766 mm

 

 

 

 

 

Evaporation temperature -25°C

 

Height (arm rest level)

840 mm

 

 

 

 

 

 

 

Overall height

 

1040 mm

 

 

 

 

 

Weight

 

 

 

 

 

 

 

 

(incl. microtome, w/o specimen cooling)

ca. 180 kg

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

24

Instruction Manual V1.2 - 11/2006

Image 24
Contents Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Symbols used in this manual and their meaning Important informationSerial- number General Designated useQualification of personnel Any other use of the instrument is considered improperIntegrated safety devices SicherheitSafety Locking the handwheelLocking UnlockingActivating the emergency stop function Deactivating the emergency stopKnife guard Site requirements Electrical connectionsDefrosting/Handling frozen tissue Frozen parts of the instrument and frozen accessoriesHandling microtome knives/blades Knife guard/handwheel lockDisinfection and cleaning Infectious/radioactive material11Removing/reinstalling the microtome MaintenanceDisplay message ‘Dry microtome’ Installation Unpacking and installationGeneral site requirements RepackingStandard delivery Further accessories Inserting the accessories Footswitch Function Models with footswitchConnecting the footswitch Prior to switching on the instrumentInstrument Properties OverviewInstrument Properties Technical Data Microtome Rotary microtome Operation Operating the instrument for the first timeSwitching on/fuses Switching onMains switch / automatic cutout for the instrument Automatic cutout for sectioning motorControl panel Key functions in control panel Display functions in control panelLock / unlock key Activates / deactivates a number of functionsY M I C R O T O M E Specimen temperature Instruments with specimen cooling only 15-minute stepsCryochamber temperature Preset counter Instruments with sectioning motor onlyPress key until menu item ‘Preset counter’ is displayed = Preset counter activatedReal time Starting time of automatic defrost cycleDuration of defrost cycle Press lock/unlock key to activate or de- activate ON/OFFStatus display StopThese parameters can be displayed via the ar- row keys Refrigeration stateSection thickness Total section thickness counter Control panel Instruments without sectioning motor Instruments with sectioning motorActivating a sectioning mode Sectioning mode 1 Intermittent strokeStart / stop motorized sectioning Press sectioning window key once more Emergency stop Functional area B all models Coarse feed keys Coarse feed ‘fast forward’ Travelling speed 1,000 μm/sTo start, press and hold Setting the section thickness Selecting trimming thicknessMotorized trimming ‘Defrosting Handling frozen tissue’ Chapter Daily operation of the instrument‘Knife guard / Handwheel lock’ Chapter Installing the 90 prism Freezing specimens with the 90 prismMobile heat extractor See .3.4 ‘Menu functions’ itemThermoblock Inserting specimen discs into the specimen headSpecimen orientation Trimming / sectioning Inserting the knife Manual trimmingSectioning Insert the knife / disposable blade into the knife holderSwitching off the instrument for a longer period of time Finishing work 5.6.1 Daily routine stepsLock handwheel Defrosting Automatic chamber defrost cycleInstruments with specimen cooling Manual chamber defrost cycle Troubleshooting, applications tips Potential problems causes and remedies Problem Causes RemediesProblem Causes Luke-warm specimen disc Problem Causes Remedies Microtome defectiveReference chart for temperature settings in minus C TissueDisinfection, cleaning and maintenance Safety instructions On disinfection and cleaningSpray disinfection with Leica Cryofect Allow to soak for 15 minutesCleaning Switch the instrument offDisinfection with conventional disinfectants Remove section waste from the chamber and dispose of it‘Disinfection and cleaning’ Chapter Reinstalling the microtome Place plastic coupling 11 on handwheel couplingUnlock handwheel Installing the replacement lamp Exchanging the fluorescent light lampRemoval Removing the sliding windowWarranty and service WarrantyTechnical service information Decommissioning and disposalElectrohydraulic height adjustment Safety instructionsAppendix Installation Maintenance and care TroubleshootingControl keys Ordering information accessories Knife holders AccessoriesSpecimen discs ConsumablesEC Declaration of Conformity Dag Graupner Managing DirectorThis equipment has been cleaned and decontaminated Decontamination Certificate masterDear Customer NB Microtome knives must be in boxesBU Return Authorisation Number