Leica CM3050 S instruction manual Problem Causes Remedies, Microtome defective

6.Troubleshooting, applications tips

Problem	Causes		Remedies
Sections tear.	-	Temperature too low for the	-	Select higher temperature and
		tissue to be sectioned.		wait for the new temperature
				to be reached.
	- Knife blunt, dirty, dusty, frosted		- Eliminate cause (--> see details
		or rusty.		on individual problems).
	- Upper edge of anti-roll plate		-	Exchange anti-roll plate.
		damaged.
	-	Hard particles in the tissue.	- If application permits, section
				at lower plane.
	-	Rear face of knife dirty.	-	Clean.

Inconsistent or insufficient	-	Microtome was not completely
specimen feed.		dry when refrigeration was
		switched on; ice build-up inside
		the micrometer mechanism.
	-	Microtome defective.

- Remove microtome from cham- ber and dry - possibly with hair- dryer (hairdryer only to be used outside the cryochamber!) or at ambient temperature.

- Call Technical Service.

Specimen disc cannot be	- Specimen disc stuck to quick-
removed.	freeze shelf or specimen head
	due to moisture on underside
	of specimen disc.

-Apply strong alcohol to contact point or heat the specimen head.

Cryostat inoperational.	-	Mains plug not properly con-		-	Check mains plug connection.
		nected.
	-	Automatic cut-out has been		-	Reconnect automatic cut-out
		triggered.			(switch instrument back on).
Sectioning motor inoperational.	-	Footswitch not connected.		-	Connect footswitch.
	- Automatic cut-out of sectioning			-	Reconnect automatic cut-out
		motor has been	trig-		(push inwards until it locks in
		gered.			place).
	-	Motor defective.		-	Call Technical Service.
No or insufficient refrigerating	-	Stopper not placed properly		-	Close drain with stopper.
performance.		into drain in bottom of cryo-
		chamber.
	-	Compressor defective.		-	Call Technical Service.
	-	Leak in cooling system.		-	Call Technical Service.

Leica CM3050 S – Cryostat 51