Leica CM3050 S instruction manual Luke-warm specimen disc

6. Troubleshooting, applications tips

Problem Causes Remedies

Anti-roll plate damaged after adjustment.

-Anti-roll plate protrudes too far beyond the knife edge. Adjust- ment was done in the direction of the knife.

-Replace anti-roll plate; in future, lift away from the knife when aligning.

-Handle anti-roll plate more carefully.

Thick/thin sections.	-	Wrong temperature for the	-	Select correct temperature.
		tissue to be sectioned.	- Wait until correct temperature
				has been reached.
	- Knife profile inappropriate for		- Use knife with different profile
		the specimen to be sectioned.		(c or d) or possible switch to
				disposable blade system.
	- Ice build-up on the knife back.		-	Remove ice.
	- Handwheel rotation not uni-		-	Adapt speed.
		form or inappropriate rotation
		speed.
	- Knife not clamped tightly		-	Check knife clamping.
		enough.
	-	Specimen disc not clamped	-	Check disc clamping.
		correctly.
	- Embedding medium poured		- Place embedding medium on
		onto cold specimen disc; spec-		luke-warm specimen disc,
		imen comes off the disc once		inside the cryochamber place
		frozen.		the specimen onto the disc and
				freeze in quick-freeze shelf.
	-	Blunt knife.	- Use different part of knife or
				replace knife.
	-	Incorrect clearance angle.	- Set correct clearance angle.
	-	Specimen dried out.	-	Prepare new specimen.

Tissue sticks to the anti-roll plate.

-Anti-roll plate too warm or incorrectly adjusted.

-Static electricity.

-Fat on corner or edge of anti- roll plate.

-Knife rusty.

-Cool or readjust anti-roll plate.

-Eliminate cause.

-Remove fat (alcohol).

-Remove rust.

Flattened sections curl up as	-	Static electricity or draft.	-	Eliminate cause.
soon as anti-roll plate is lifted	-	Anti-roll plate too warm.	-	Cool anti-roll plate.
from the knife.

50 Instruction Manual V1.1 02/2000