Leica Comprehensive Operation Guide and Menu Functions for CM3050 Refrigeration Settings

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5.Operation

5.3.4Menu functions: setting parameters of refrigeration, time and preset counter

Once the instrument has gone through the initial sequence of indications, you can go on to the menu functions to select and/or change instru- ment parameters.

If display is locked:

Press key until display background lights up.

Next, press the menu key. With each key stroke the following parameters will be displayed (dis- play sequence as listed):

1.Specimen temperature

(in instruments with specimen cooling only) (-10 - -50 °C)

2.Cryochamber temperature (0 - -40 °C)

3.Selected number of sections - preset counter

(ON/OFF and setting selection from 1 to 999)

1.Specimen temperature:

(instruments with specimen cooling only)

Press key until menu item ‘Set specimen temperature’ is displayed:

S E T T E M P O T - - - ° C

- -

Press key briefly.

The line in the specimen temperature field is replaced by an asterisk.

S E T T E M P O T - - - ° C

-

From this point on the refrigeration sys- tem is activated and the desired set temperature can be selected with one of the arrow keys.

Press arrow key and release upon reaching the desired value.

C

T

.

.

.

. °

C

 

 

O

T

-

3

5

°

C

￿

￿

H

O

M

E

 

 

 

 

 

 

 

 

-

4.Maximum specimen temperature (ON/OFF)

5.Real time

(in 1-minute steps)

6.Defrost time (starting time of automatic cryochamber defrosting)

(in 15-minute steps)

7.Duration of defrost cycle (cryochamber defrosting) (6 to 12 minutes duration)

Menu items 5 and 6 displayed on 24 hour clock.

Note on the arrow key function:

Each time one of the arrow keys is pressed briefly, the value displayed in the menu will be changed one step up or down.

If an arrow key is pressed and held, the value will change continually (auto-repeat function). When holding the key, scrolling speed will in- crease after a while.

As soon as a value is displayed, it is at the same time automatically stored.

Leica CM 3050 S – Cryostat

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Contents Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Serial- number Symbols used in this manual and their meaningImportant information Designated use GeneralQualification of personnel Any other use of the instrument is considered improperSicherheit Integrated safety devicesLocking the handwheel SafetyLocking UnlockingKnife guard Activating the emergency stop functionDeactivating the emergency stop Electrical connections Site requirementsFrozen parts of the instrument and frozen accessories Defrosting/Handling frozen tissueHandling microtome knives/blades Knife guard/handwheel lockInfectious/radioactive material Disinfection and cleaningDisplay message ‘Dry microtome’ 11Removing/reinstalling the microtomeMaintenance Unpacking and installation InstallationGeneral site requirements RepackingStandard delivery Further accessories Inserting the accessories Models with footswitch Footswitch FunctionConnecting the footswitch Prior to switching on the instrumentOverview Instrument PropertiesInstrument Properties Technical Data Microtome Rotary microtome Operating the instrument for the first time OperationSwitching on Switching on/fusesMains switch / automatic cutout for the instrument Automatic cutout for sectioning motorDisplay functions in control panel Control panel Key functions in control panelLock / unlock key Activates / deactivates a number of functionsY M I C R O T O M E 15-minute steps Specimen temperature Instruments with specimen cooling onlyPreset counter Instruments with sectioning motor only Cryochamber temperaturePress key until menu item ‘Preset counter’ is displayed = Preset counter activatedStarting time of automatic defrost cycle Real timeDuration of defrost cycle Press lock/unlock key to activate or de- activate ON/OFFStop Status displaySection thickness Total section thickness counter These parameters can be displayed via the ar- row keysRefrigeration state Instruments with sectioning motor Control panel Instruments without sectioning motorStart / stop motorized sectioning Activating a sectioning modeSectioning mode 1 Intermittent stroke Press sectioning window key once more Emergency stop To start, press and hold Functional area B all models Coarse feed keysCoarse feed ‘fast forward’ Travelling speed 1,000 μm/s Motorized trimming Setting the section thicknessSelecting trimming thickness ‘Knife guard / Handwheel lock’ Chapter ‘Defrosting Handling frozen tissue’ ChapterDaily operation of the instrument Freezing specimens with the 90 prism Installing the 90 prismMobile heat extractor See .3.4 ‘Menu functions’ itemSpecimen orientation ThermoblockInserting specimen discs into the specimen head Manual trimming Trimming / sectioning Inserting the knifeSectioning Insert the knife / disposable blade into the knife holderLock handwheel Switching off the instrument for a longer period of timeFinishing work 5.6.1 Daily routine steps Instruments with specimen cooling DefrostingAutomatic chamber defrost cycle Manual chamber defrost cycle Troubleshooting, applications tips Remedies Potential problems causes and remedies Problem CausesProblem Causes Luke-warm specimen disc Microtome defective Problem Causes RemediesTissue Reference chart for temperature settings in minus CSafety instructions On disinfection and cleaning Disinfection, cleaning and maintenanceSpray disinfection with Leica Cryofect Allow to soak for 15 minutesSwitch the instrument off CleaningDisinfection with conventional disinfectants Remove section waste from the chamber and dispose of it‘Disinfection and cleaning’ Chapter Unlock handwheel Reinstalling the microtomePlace plastic coupling 11 on handwheel coupling Exchanging the fluorescent light lamp Installing the replacement lampRemoval Removing the sliding windowWarranty Warranty and serviceTechnical service information Decommissioning and disposalAppendix Electrohydraulic height adjustmentSafety instructions Installation Control keys Maintenance and careTroubleshooting Accessories Ordering information accessories Knife holdersSpecimen discs ConsumablesDag Graupner Managing Director EC Declaration of ConformityDecontamination Certificate master This equipment has been cleaned and decontaminatedDear Customer NB Microtome knives must be in boxesBU Return Authorisation Number