Leica CM3050 S Switching off the instrument for a longer period of time, Lock handwheel

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5.Operation

5.6Finishing work 5.6.1 Daily routine steps

Remove all specimens from the cry- ochamber and store safely! See also corresponding safety instruc- tions in chapter 2.3.7 ‘Defrosting / handling frozen tissue’!

•Lock handwheel.

•Remove knife from knife holder, disinfect, dry (see chapter 7 ‘Disinfection, cleaning and maintenance’) and store in the knife case.

•Use a cold brush to sweep frozen sectioning waste into the section waste tray.

•Empty section waste tray (dispose of sec- tioning waste according to the applicable regulations on disposal of special category waste!).

•If necessary, carry out a complete disinfection procedure (see chapter 7 ‘Disinfection, cleaning and maintenance’).

•Remove all specimens from the chamber and store safely.

•Close sliding window.

•Switch off cryochamber illumination.

•Use lock/unlock key to lock display in control panel 1 (Display background dark = locked):

C	T	.	.	.	. °	C		O	T	.	.	.	.	°	C
					1	2	:	0	0

Do not switch off mains switch / automatic cutout (see chapter 5.2 ‘Switching on / fuses’)!

5.6.2Switching off the instrument for a longer period of time

If you do not plan to use the instrument for an extended period of time, it can be switched off.

However, since this involves a lot of work (see below), it should be considered carefully whether switching off the instrument is really the better option.

If the instrument is switched off, the microtome must be removed. Then the microtome, cryo- chamber and all accessories must be disinfect- ed and dried!

•For that purpose, follow the instructions in chapters 7.1 through 7.5.3.

44	Instruction Manual V1.2 - 11/2006

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Contents Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Serial- number Symbols used in this manual and their meaningImportant information General Designated useQualification of personnel Any other use of the instrument is considered improperIntegrated safety devices SicherheitSafety Locking the handwheelLocking UnlockingKnife guard Activating the emergency stop functionDeactivating the emergency stop Site requirements Electrical connectionsDefrosting/Handling frozen tissue Frozen parts of the instrument and frozen accessoriesHandling microtome knives/blades Knife guard/handwheel lockDisinfection and cleaning Infectious/radioactive materialDisplay message ‘Dry microtome’ 11Removing/reinstalling the microtomeMaintenance Installation Unpacking and installationGeneral site requirements RepackingStandard delivery Further accessories Inserting the accessories Footswitch Function Models with footswitchConnecting the footswitch Prior to switching on the instrumentInstrument Properties OverviewInstrument Properties Technical Data Microtome Rotary microtome Operation Operating the instrument for the first timeSwitching on/fuses Switching onMains switch / automatic cutout for the instrument Automatic cutout for sectioning motorControl panel Key functions in control panel Display functions in control panelLock / unlock key Activates / deactivates a number of functionsY M I C R O T O M E Specimen temperature Instruments with specimen cooling only 15-minute stepsCryochamber temperature Preset counter Instruments with sectioning motor onlyPress key until menu item ‘Preset counter’ is displayed = Preset counter activatedReal time Starting time of automatic defrost cycleDuration of defrost cycle Press lock/unlock key to activate or de- activate ON/OFFStatus display StopSection thickness Total section thickness counter These parameters can be displayed via the ar- row keysRefrigeration state Control panel Instruments without sectioning motor Instruments with sectioning motorStart / stop motorized sectioning Activating a sectioning modeSectioning mode 1 Intermittent stroke Press sectioning window key once more Emergency stop To start, press and hold Functional area B all models Coarse feed keysCoarse feed ‘fast forward’ Travelling speed 1,000 μm/s Motorized trimming Setting the section thicknessSelecting trimming thickness ‘Knife guard / Handwheel lock’ Chapter ‘Defrosting Handling frozen tissue’ ChapterDaily operation of the instrument Installing the 90 prism Freezing specimens with the 90 prismMobile heat extractor See .3.4 ‘Menu functions’ itemSpecimen orientation ThermoblockInserting specimen discs into the specimen head Trimming / sectioning Inserting the knife Manual trimmingSectioning Insert the knife / disposable blade into the knife holderLock handwheel Switching off the instrument for a longer period of timeFinishing work 5.6.1 Daily routine steps Instruments with specimen cooling DefrostingAutomatic chamber defrost cycle Manual chamber defrost cycle Troubleshooting, applications tips Potential problems causes and remedies Problem Causes RemediesProblem Causes Luke-warm specimen disc Problem Causes Remedies Microtome defectiveReference chart for temperature settings in minus C TissueDisinfection, cleaning and maintenance Safety instructions On disinfection and cleaningSpray disinfection with Leica Cryofect Allow to soak for 15 minutesCleaning Switch the instrument offDisinfection with conventional disinfectants Remove section waste from the chamber and dispose of it‘Disinfection and cleaning’ Chapter Unlock handwheel Reinstalling the microtomePlace plastic coupling 11 on handwheel coupling Installing the replacement lamp Exchanging the fluorescent light lampRemoval Removing the sliding windowWarranty and service WarrantyTechnical service information Decommissioning and disposalAppendix Electrohydraulic height adjustmentSafety instructions Installation Control keys Maintenance and careTroubleshooting Ordering information accessories Knife holders AccessoriesSpecimen discs ConsumablesEC Declaration of Conformity Dag Graupner Managing DirectorThis equipment has been cleaned and decontaminated Decontamination Certificate masterDear Customer NB Microtome knives must be in boxesBU Return Authorisation Number