Leica CM3050 S Reinstalling the microtome, Place plastic coupling 11 on handwheel coupling

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7.Disinfection, cleaning and maintenance

•Remove plug of temperature sensor (7).

•Remove plug of coarse feed motor:

Pull bushing backward (arrow 1) and hold. Place your thumb between plug (6) and socket at the microtome (arrow 2).

To remove plug (6) from microtome, pull it away from the microtome while at the same time carrying out a slight jogging movement

(arrows 3).

7

9

6

8

	2
3	3
3
	1

•Loosen screws (8) with Allen key.

•Lift microtome a little and pull slightly to the left.

•This loosens the plastic coupling (11) which connects the two axles.

• Remove the microtome from the chamber.

7.5.3 Reinstalling the microtome

When reinstalling the microtome make sure to observe the following safety instructions:

•‘Removing / reinstalling the microtome’ (Chapter 2.3.11)

•Display message ‘Dry Microtome’ (Chapter 2.3.12)

•Lock handwheel in lower position.

•Place microtome in chamber.

•Put one drop of cryostat oil per surface on the contact surfaces of plastic coupling (11) and handwheel coupling (12).

•Place plastic coupling (11) on handwheel coupling (12).

•Push microtome slightly towards the right until axle (9) fits tightly into coupling (11).

•Unlock handwheel.

•Adjust the parts into each other by rotating the handwheel slightly backward and forth.

•Lock handwheel again in lower position.

•Tighten screws (8).

•Reconnect plugs of coarse feed motor (6) and temperature sensor (7).

•Mount specimen head (in reverse order as described on previous page).

•Insert accessories (storage shelf, section waste tray etc.).

•Insert sliding window.

•Connect instrument to mains and switch back on.

56	Instruction Manual V1.2 - 11/2006

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Contents Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Serial- number Symbols used in this manual and their meaningImportant information General Designated useQualification of personnel Any other use of the instrument is considered improperIntegrated safety devices SicherheitSafety Locking the handwheelLocking UnlockingKnife guard Activating the emergency stop functionDeactivating the emergency stop Site requirements Electrical connectionsDefrosting/Handling frozen tissue Frozen parts of the instrument and frozen accessoriesHandling microtome knives/blades Knife guard/handwheel lockDisinfection and cleaning Infectious/radioactive materialDisplay message ‘Dry microtome’ 11Removing/reinstalling the microtomeMaintenance Installation Unpacking and installationGeneral site requirements RepackingStandard delivery Further accessories Inserting the accessories Footswitch Function Models with footswitchConnecting the footswitch Prior to switching on the instrumentInstrument Properties OverviewInstrument Properties Technical Data Microtome Rotary microtome Operation Operating the instrument for the first timeSwitching on/fuses Switching onMains switch / automatic cutout for the instrument Automatic cutout for sectioning motorControl panel Key functions in control panel Display functions in control panelLock / unlock key Activates / deactivates a number of functionsY M I C R O T O M E Specimen temperature Instruments with specimen cooling only 15-minute stepsCryochamber temperature Preset counter Instruments with sectioning motor onlyPress key until menu item ‘Preset counter’ is displayed = Preset counter activatedReal time Starting time of automatic defrost cycleDuration of defrost cycle Press lock/unlock key to activate or de- activate ON/OFFStatus display StopSection thickness Total section thickness counter These parameters can be displayed via the ar- row keysRefrigeration state Control panel Instruments without sectioning motor Instruments with sectioning motorStart / stop motorized sectioning Activating a sectioning modeSectioning mode 1 Intermittent stroke Press sectioning window key once more Emergency stop To start, press and hold Functional area B all models Coarse feed keysCoarse feed ‘fast forward’ Travelling speed 1,000 μm/s Motorized trimming Setting the section thicknessSelecting trimming thickness ‘Knife guard / Handwheel lock’ Chapter ‘Defrosting Handling frozen tissue’ ChapterDaily operation of the instrument Installing the 90 prism Freezing specimens with the 90 prismMobile heat extractor See .3.4 ‘Menu functions’ itemSpecimen orientation ThermoblockInserting specimen discs into the specimen head Trimming / sectioning Inserting the knife Manual trimmingSectioning Insert the knife / disposable blade into the knife holderLock handwheel Switching off the instrument for a longer period of timeFinishing work 5.6.1 Daily routine steps Instruments with specimen cooling DefrostingAutomatic chamber defrost cycle Manual chamber defrost cycle Troubleshooting, applications tips Potential problems causes and remedies Problem Causes RemediesProblem Causes Luke-warm specimen disc Problem Causes Remedies Microtome defectiveReference chart for temperature settings in minus C TissueDisinfection, cleaning and maintenance Safety instructions On disinfection and cleaningSpray disinfection with Leica Cryofect Allow to soak for 15 minutesCleaning Switch the instrument offDisinfection with conventional disinfectants Remove section waste from the chamber and dispose of it‘Disinfection and cleaning’ Chapter Unlock handwheel Reinstalling the microtomePlace plastic coupling 11 on handwheel coupling Installing the replacement lamp Exchanging the fluorescent light lampRemoval Removing the sliding windowWarranty and service WarrantyTechnical service information Decommissioning and disposalAppendix Electrohydraulic height adjustmentSafety instructions Installation Control keys Maintenance and careTroubleshooting Ordering information accessories Knife holders AccessoriesSpecimen discs ConsumablesEC Declaration of Conformity Dag Graupner Managing DirectorThis equipment has been cleaned and decontaminated Decontamination Certificate masterDear Customer NB Microtome knives must be in boxesBU Return Authorisation Number