Leica CM3050 S Important information, Symbols used in this manual and their meaning

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1.Important information

The chapters of the Leica CM3050 S cryostat instruction manual:

Chapter 1 Structure of the manual:

•Table of contents

•Important information on this manual

Chapter 2 Safety

•Read this chapter before operating the instrument!

Chapter 3 Installation

•Unpacking and installation

•Standard delivery, information on accessories

Chapter 4 Instrument properties

•Overview

•Technical data

Chapter 5 Operation

•Controls

•Setup, daily use and shut down

Chapter 6 Trouble shooting and applications tips

•Operating errors

•Display error messages

•Temperature reference chart

Chapter 7 Disinfection, cleaning and maintenance

Chapter 8 Warranty and service

Chapter 9 Electrohydraulic height adjustment Ordering information accessories EC Declaration of Conformity

1.1Symbols used in this manual and their meaning

Warnings

appear in a grey box and are marked by a warning triangle .

Notes

i.e. important user information appears in a grey box and is marked

by an information symbol.

(5)Figures in brackets refer to item

(Fig.5) numbers in drawings or to the drawings themselves.

Instrument type:

All information given in this instruction manual applies only to the instrument type indicated on the title page.

A name plate, indicating the instrument serial number, is attached to the back of the instru- ment.

Required in- formation for all inquiries:

For any inquir- ies please specify:

•Instrument type

•Serial- number

Leica CM 3050 S – Cryostat

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Contents Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Important information Symbols used in this manual and their meaningSerial- number Any other use of the instrument is considered improper GeneralDesignated use Qualification of personnelSicherheit Integrated safety devicesUnlocking SafetyLocking the handwheel LockingDeactivating the emergency stop Activating the emergency stop functionKnife guard Electrical connections Site requirementsKnife guard/handwheel lock Defrosting/Handling frozen tissueFrozen parts of the instrument and frozen accessories Handling microtome knives/bladesInfectious/radioactive material Disinfection and cleaningMaintenance 11Removing/reinstalling the microtomeDisplay message ‘Dry microtome’ Repacking InstallationUnpacking and installation General site requirementsStandard delivery Further accessories Inserting the accessories Prior to switching on the instrument Footswitch FunctionModels with footswitch Connecting the footswitchOverview Instrument PropertiesInstrument Properties Technical Data Microtome Rotary microtome Operating the instrument for the first time OperationAutomatic cutout for sectioning motor Switching on/fusesSwitching on Mains switch / automatic cutout for the instrumentActivates / deactivates a number of functions Control panel Key functions in control panelDisplay functions in control panel Lock / unlock keyY M I C R O T O M E 15-minute steps Specimen temperature Instruments with specimen cooling only= Preset counter activated Cryochamber temperaturePreset counter Instruments with sectioning motor only Press key until menu item ‘Preset counter’ is displayedPress lock/unlock key to activate or de- activate ON/OFF Real timeStarting time of automatic defrost cycle Duration of defrost cycleStop Status displayRefrigeration state These parameters can be displayed via the ar- row keysSection thickness Total section thickness counter Instruments with sectioning motor Control panel Instruments without sectioning motorSectioning mode 1 Intermittent stroke Activating a sectioning modeStart / stop motorized sectioning Press sectioning window key once more Emergency stop Coarse feed ‘fast forward’ Travelling speed 1,000 μm/s Functional area B all models Coarse feed keysTo start, press and hold Selecting trimming thickness Setting the section thicknessMotorized trimming Daily operation of the instrument ‘Defrosting Handling frozen tissue’ Chapter‘Knife guard / Handwheel lock’ Chapter See .3.4 ‘Menu functions’ item Installing the 90 prismFreezing specimens with the 90 prism Mobile heat extractorInserting specimen discs into the specimen head ThermoblockSpecimen orientation Insert the knife / disposable blade into the knife holder Trimming / sectioning Inserting the knifeManual trimming SectioningFinishing work 5.6.1 Daily routine steps Switching off the instrument for a longer period of timeLock handwheel Automatic chamber defrost cycle DefrostingInstruments with specimen cooling Manual chamber defrost cycle Troubleshooting, applications tips Remedies Potential problems causes and remedies Problem CausesProblem Causes Luke-warm specimen disc Microtome defective Problem Causes RemediesTissue Reference chart for temperature settings in minus CAllow to soak for 15 minutes Disinfection, cleaning and maintenanceSafety instructions On disinfection and cleaning Spray disinfection with Leica CryofectRemove section waste from the chamber and dispose of it CleaningSwitch the instrument off Disinfection with conventional disinfectants‘Disinfection and cleaning’ Chapter Place plastic coupling 11 on handwheel coupling Reinstalling the microtomeUnlock handwheel Removing the sliding window Installing the replacement lampExchanging the fluorescent light lamp RemovalDecommissioning and disposal Warranty and serviceWarranty Technical service informationSafety instructions Electrohydraulic height adjustmentAppendix Installation Troubleshooting Maintenance and careControl keys Consumables Ordering information accessories Knife holdersAccessories Specimen discsDag Graupner Managing Director EC Declaration of ConformityNB Microtome knives must be in boxes This equipment has been cleaned and decontaminatedDecontamination Certificate master Dear CustomerBU Return Authorisation Number