Leica CM3050 S 11Removing/reinstalling the microtome, Display message ‘Dry microtome’

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2.Safety

2.3.11Removing/reinstalling the microtome

Before removing the microtome:

Switch instrument off.

Unplug from mains.

Place handwheel grip in lowest position and lock.

-->When removing the microtome, the specimen head must always be lok- ked in the lowest position. Otherwise the upper part of the slot cover might be bent and consequently damaged!

When removing the microtome:

Wear gloves when removing the micro- tome while it is still frozen.

-->Risk of frost bite!

On instruments with specimen cooling:

do not distort the refrigerating tube!

If distorted it might break, causing ex-

tremely cold refrigerant to escape.

- -

>Risk of frost bite!

Before reinstalling the microtome:

• Microtome must be completely dry.

--

>Humidity in the interior of the micro-

 

tome freezes and causes

 

microtome

malfunctions and/or

 

damage to the

microtome.

 

All accessories/tools removed from the cryochamber must be thoroughly dry be- fore putting them back into the chamber!

-->Risk of icing!

2.3.12 Display message ‘Dry microtome’

If the error message ‘Dry Microtome’ is dis- played in control panel 1, the following has happened:

Cryochamber refrigeration has been in- terrupted for an extended period of time (e.g. power failure), causing the chamber temperature to rise into the positive digits.

If this message appears, do not switch on the instrument but remove the microtome from the chamber, disinfect, if necessary, and dry thoroughly before reinstalling it into the chamber (see chapter 7.1 to 7.5).

2.3.13 Maintenance

Only technical service engineers authorized by Leica may access the internal compo- nents of the instrument for service and re- pair.

The fluorescent light lamp (chamber illumi- nation), unless broken or splintered, can be replaced by the user:

Switch off mains switch!

Unplug the instrument from mains!

If the lamp is broken or splintered:

Have lamp replaced by Leica Technical Service!

-->Risk of injury!

Use only those replacement lamps that cor- respond to technical specification (see chapter 4.2 ‘Technical Data’).

Leica CM3050 S – Cryostat

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Contents Leica CM3050 S Page Important note Table of Contents Table of Contents EC Declaration of Conformity Symbols used in this manual and their meaning Important informationSerial- number Any other use of the instrument is considered improper GeneralDesignated use Qualification of personnelSicherheit Integrated safety devicesUnlocking SafetyLocking the handwheel LockingActivating the emergency stop function Deactivating the emergency stopKnife guard Electrical connections Site requirementsKnife guard/handwheel lock Defrosting/Handling frozen tissueFrozen parts of the instrument and frozen accessories Handling microtome knives/bladesInfectious/radioactive material Disinfection and cleaning11Removing/reinstalling the microtome MaintenanceDisplay message ‘Dry microtome’ Repacking InstallationUnpacking and installation General site requirementsStandard delivery Further accessories Inserting the accessories Prior to switching on the instrument Footswitch FunctionModels with footswitch Connecting the footswitchOverview Instrument PropertiesInstrument Properties Technical Data Microtome Rotary microtome Operating the instrument for the first time OperationAutomatic cutout for sectioning motor Switching on/fusesSwitching on Mains switch / automatic cutout for the instrumentActivates / deactivates a number of functions Control panel Key functions in control panelDisplay functions in control panel Lock / unlock keyY M I C R O T O M E 15-minute steps Specimen temperature Instruments with specimen cooling only= Preset counter activated Cryochamber temperaturePreset counter Instruments with sectioning motor only Press key until menu item ‘Preset counter’ is displayedPress lock/unlock key to activate or de- activate ON/OFF Real timeStarting time of automatic defrost cycle Duration of defrost cycleStop Status displayThese parameters can be displayed via the ar- row keys Refrigeration stateSection thickness Total section thickness counter Instruments with sectioning motor Control panel Instruments without sectioning motorActivating a sectioning mode Sectioning mode 1 Intermittent strokeStart / stop motorized sectioning Press sectioning window key once more Emergency stop Functional area B all models Coarse feed keys Coarse feed ‘fast forward’ Travelling speed 1,000 μm/sTo start, press and hold Setting the section thickness Selecting trimming thicknessMotorized trimming ‘Defrosting Handling frozen tissue’ Chapter Daily operation of the instrument‘Knife guard / Handwheel lock’ Chapter See .3.4 ‘Menu functions’ item Installing the 90 prismFreezing specimens with the 90 prism Mobile heat extractorThermoblock Inserting specimen discs into the specimen headSpecimen orientation Insert the knife / disposable blade into the knife holder Trimming / sectioning Inserting the knifeManual trimming SectioningSwitching off the instrument for a longer period of time Finishing work 5.6.1 Daily routine stepsLock handwheel Defrosting Automatic chamber defrost cycleInstruments with specimen cooling Manual chamber defrost cycle Troubleshooting, applications tips Remedies Potential problems causes and remedies Problem CausesProblem Causes Luke-warm specimen disc Microtome defective Problem Causes RemediesTissue Reference chart for temperature settings in minus CAllow to soak for 15 minutes Disinfection, cleaning and maintenanceSafety instructions On disinfection and cleaning Spray disinfection with Leica CryofectRemove section waste from the chamber and dispose of it CleaningSwitch the instrument off Disinfection with conventional disinfectants‘Disinfection and cleaning’ Chapter Reinstalling the microtome Place plastic coupling 11 on handwheel couplingUnlock handwheel Removing the sliding window Installing the replacement lampExchanging the fluorescent light lamp RemovalDecommissioning and disposal Warranty and serviceWarranty Technical service informationElectrohydraulic height adjustment Safety instructionsAppendix Installation Maintenance and care TroubleshootingControl keys Consumables Ordering information accessories Knife holdersAccessories Specimen discsDag Graupner Managing Director EC Declaration of ConformityNB Microtome knives must be in boxes This equipment has been cleaned and decontaminatedDecontamination Certificate master Dear CustomerBU Return Authorisation Number